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Development of a Soft X-ray Microprobe for Radiobiology Studies

100%

Appuhamilage I., Adjei D., Alatabi S., Alnaimi R., Michette A., Pfauntsch S.

vol. 125

issue 4

882-885

The King's College London (KCL) first X-ray microprobe (MKI) and the third generation microfocus X-ray sources (MKIII) are intended to be used for various applications including the study of physical and biological interactions at the atomic and molecular scales. The microfocus ultra-soft X-ray sources (MKI and MKIII) with interchangeable targets will provide a superior spatial resolution (a focal spot a few hundreds of nanometres in diameter can be achieved) and the control of the dose delivered to irradiated cells. This will require characterization of the spectra and intensities of the source, measurements of the focus intensities and spot sizes of suitable X-ray optics such as zone plates, grazing incidence microstructured optical arrays and multilayer mirrors.

Applications of a Compact "Water Window" Source for Investigations of Nanostructures Using SXR Microscope

84%

Torrisi A., Wachulak P., Fahad Nawaz M., Bartnik A., Adjei D., Vondrová Š., Turňová J., Jančarek A., Fiedorowicz H.

Acta Physica Polonica A

2016

vol. 129

issue 2

169-171

A compact soft X-ray microscope based on a nitrogen double-stream gas puff target soft X-ray source, operating at He-like nitrogen spectral line at the wavelength of λ =2.88 nm is presented. The desk-top size microscope was successfully demonstrated in transmission mode using the Fresnel zone-plate objective and it is suitable for soft X-ray source microscopy in the "water window" spectral range (λ = 2.3÷ 4.4 nm). Details about the soft X-ray source source, the microscope and an example of application in the biomedical field are shown and discussed.

Contact Microscopy using a Compact Laser Produced Plasma Soft X-Ray Source

68%

Ayele M., Czwartos J., Adjei D., Wachulak P., Ahad I., Bartnik A., Wegrzynski Ł., Szczurek M., Jarocki R., Fiedorowicz H., Lekka M., Pogoda K., Gostek J.

Acta Physica Polonica A

2016

vol. 129

issue 2

237-240

Soft X-ray contact microscopy potentially allows imaging of wet living biological specimens at a spatial resolution higher than optical microscopy and without many of the constraints of scanning electron microscopy. In this paper, we present the development of a laboratory scale contact microscope that uses a laser produced plasma soft X-ray source. The source is based on a double-stream gas-puff target approach and it operates in the "water window" spectral range which enables to capture images of biological samples with a natural contrast. In the preliminary experiments the contact microscope system has been used for imaging of fixed and dried non-malignant HCV29 human bladder cell lines cultured on polymethyl methacrylate photoresists. The samples were exposed with 150 pulses of soft X-rays as an initial test to demonstrate the possibility of image formation. The soft X-ray contact images registered in the photoresists exhibit high resolution in the atomic force microscopy topography which indicates the potential application of soft X-ray contact microscopy in life science to examine small features as small as few tens of nm. The technique could also be used for living cell imaging with further optimization of the microscope system and development of a special specimen holder. The details of the soft X-ray contact microscopy technique and the experimental results are presented and discussed.

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3 Acta Physica Polonica A

2 2016

1 2014

3 Adjei D.

2 Bartnik A.

2 Fiedorowicz H.

2 Wachulak P.

1 Ahad I.

1 Alatabi S.

1 Alnaimi R.

1 Appuhamilage I.

1 Ayele M.

1 Czwartos J.

1 Fahad Nawaz M.

1 Gostek J.

1 Jančarek A.

1 Jarocki R.

1 Lekka M.

1 Michette A.

1 Pfauntsch S.

1 Pogoda K.

1 Szczurek M.

1 Torrisi A.

1 Turňová J.

1 Vondrová Š.

1 Wegrzynski Ł.

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Development of a Soft X-ray Microprobe for Radiobiology Studies

Applications of a Compact "Water Window" Source for Investigations of Nanostructures Using SXR Microscope

Contact Microscopy using a Compact Laser Produced Plasma Soft X-Ray Source