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1

Reproductive performance of a transgenic boar carrying the bovine growth hormone gene (bGH)

100%
Rejduch B., Slota E., Sysa P., Rozycki M.
Journal of Applied Genetics
|
2002
|
vol. 43
|
issue 3
337-341
EN
The aim of this study was to obtain information on the possible influence of the bovine growth hormone gene (bGH) on gametogenesis and reproductive parameters of a 2-year-old Polish Landrace transgenic boar. The bGH gene construct had been introduced into the zygote of the boar with the use of the microinjection technique. On the basis of the available documentation we established that the fertility of the investigated transgenic (bGH) boar was low in comparison with other animals of the Polish Landrace breed, with a poor libido, ineffective matings and, on average, 3 live piglets less per litter. Samples of testis tissue from the boar were obtained after castration. In total, we observed 100 spermatocytes and all of them had normally paired bivalents. It is possible that the boar?s lower fertility was caused by some, as yet unknown factor.
2

Known mutation (A3072G) in intron 3 of the IGF2 gene is associated with growth and carcass composition in Polish pig breeds

88%
Oczkowicz M., Tyra M., Walinowicz K., Rozycki M., Rejduch B.
Journal of Applied Genetics
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2009
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vol. 50
|
issue 3
257-259
EN
IGF2 is one of the genes that control muscle development. Moreover, IGF2 is imprinted, as only the paternal allele is expressed in the offspring. Using real-time PCR for IGF2 genotyping (Carrodegous et al. 2005), we evaluated the frequency of the IGF2 A3072G mutation (Van Laere et al. 2003) in pigs: Polish Landrace (PL, N = 271) and Large White (LW, N = 267). Our results are consistent with previous reports, showing that the A allele is common in breeds subjected to strong selection for lean meat content (A allele frequency was 0.79 in LW and 0.69 in PL). Moreover, we compared body composition, growth performance and meat quality traits in pigs carrying opposite genotypes (A/A and G/G) in the IGF2 gene. The association study revealed that the A allele increases the weight of loin (WL) (additive gene effect = 450+_50 g in LW and 213+_64g in PL), weight of ham (WH) (544+_48 g in LW and 302+_72 g in PL), loin eye area (LEA) (4.9+_0.46 cm2 in LW and 2.1 +_0.95 cm2 in PL), carcass meat percentage (CP) (3.12+_0.27% in LW and 1.89+_0.47% in PL), and decreases average backfat thickness (ABF) (-0.2?0.036 cm in LW and -0.2+_0.048 cm in PL). Additionally, in PL, the A allele increases the weight of tenderloin (WT) (11?0.01 g), average daily gain (ADG) (30.7+_17.29 g), and decreases feed intake (F) (-121+_45 g) and days of feeding (DF) (-3.5+_2.08 days). No significant effects were observed for meat quality traits. Our results suggest that selection based on the IGF2 mutation in Poland may be very useful in PL and LW pigs, where the G allele is still relatively frequent.
3

Polymorphisms in coding and regulatory regions of the porcine MYF6 and MYOG genes and expression of the MYF6 gene in m. longissimus dorsi versus productive traits in pigs

76%
Wyszynska-Koko J., Pierzchala M., Flisikowski K., Kamyczek M., Rozycki M., Kuryl J.
Journal of Applied Genetics
|
2006
|
vol. 47
|
issue 2
131-138
EN
MYOG and MYF6 belong to the MyoD gene family. They code for the bHLH transcription factors playing a key role in later stages of myogenesis: differentiation and maturation of myotubes. Three SNPs in porcine MYF6 and two in porcine MYOG were analysed in order to establish associations with chosen carcass quality and growth rate traits in Polish Landrace, Polish Large White and line 990 sows. No statistically significant effect of SNP in the promoter region of the MYF6 gene on its expression measured on mRNA level was found. Associations between the genotype at the MYF6 locus and carcass quality traits appeared to be breed-dependent. The C allele in the case of SNP in the promoter region and GC haplotype in exon 1 were advantageous for right carcass side weight in Polish Landrace sows and disadvantageous for this trait in Polish Large White sows. These gene variants were also the most advantageous for loin and ham weight in sows of line 990. The mutation in exon 1 of the MYOG gene had no statistically significant association with carcass quality traits and the mutation in the 3'-flanking region had the breed-dependent effect as well. These results suggest that SNPs analysed in this study are not causative mutations, but can be considered as markers of some other, still unrevealed genetic polymorphism that influences the physiological processes and phenotypic traits considered in this study.
4

Transgenic pigs: production and genetic modification

76%
Smorag Z., Jura J., Kopchick J. J., Gajda B., Skrzyszowska M., Rozycki M., Pasieka J.
Biotechnologia
|
1998
|
issue 2
145-152
EN
This paper presents the methods of transgenic pigs production and the results based on the long experience of the authors in this area. Moreover, the trends and current issues of transgenic modification in pigs are discussed.
5

Performance of transgenic pigs produced with the use of two different growth hormone gene constructs

64%
Rozycki M., Smorag Z., Kopchick J., Chen W. Y., Jura J., Pasieka J., Orzechowska B., Gajda B., Skrzyszowska M.
Journal of Applied Genetics
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1999
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vol. 40
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issue 1
29-37
EN
Four transgenic pigs, produced with the use of two different gene constructs containing the bovine growth hormone coding gene - Mt-bGH-10D6 (wild type) and Mt-bGH-M8 (mutated), were used to produce the F1 generation to investigate their performance traits. No differences were observed in fattening and slaughter traits between transgenic and non-transgenic pigs. It was found that the weight of ham proper and loin eye area was significantly higher in carriers of Mt-bGH-10D6 gene constructs.
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