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The in vitro synthesis of diosgenin mono- and diglucoside by enzyme preparations from Solanum melongena leaves

100%
Pączkowski C., Wojciechowski Z. A.
Acta Biochimica Polonica
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1993
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vol. 40
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issue 1
141-143
2
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Production of oleanolic acid glycosides by hairy root established cultures of Calendula officinalis L.

81%
Długosz M., Wiktorowska E., Wiśniewska A., Pączkowski C.
Acta Biochimica Polonica
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2013
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vol. 60
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issue 3
467-473
EN
In order to initiate hairy root culture initiation cotyledons and hypocotyls of Calendula officinalis L. were infected with Agrobacterium rhizogenes strain ATCC 15834 or the same strain containing pCAMBIA 1381Z vector with β-glucuronidase reporter gene under control of promoter of NIK (Nematode Induced Kinase) gene. The efficiency of induction of hairy roots reached 33.8% for cotyledons and 66.6% for hypocotyls together for both transformation experiments. Finally, eight control and nine modified lines were established as a long-term culture. The hairy root cultures showed the ability to synthesize oleanolic acid mainly (97%) as glycosides; control lines contained it at the average 8.42 mg · g-1 dry weight in tissue and 0.23 mg · dm-3 in medium; modified lines: 4.59 mg · g-1 for the tissue, and 0.48 mg · dm-3 for the medium. Additionally lines showed high positive correlation between dry/fresh weight and oleanolic acid concentration in tissue. Using the Killiani mixture in acidic hydrolysis of oleanolic acid glycosides released free aglycones that were partially acetylated in such conditions.
3
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UDP-glucose:solasodine glucosyltranslerase from eggplant (Solanum melongena L.) leaves: Partial purification and characterization

81%
Pączkowski C., Kalinowska M., Wojciechowski Z. A.
Acta Biochimica Polonica
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1997
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vol. 44
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issue 1
43-53
4
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Evidence for essential histidine and dicarboxylic amino-acid residues in the active site of UDP-glucose : solasodine glucosyltransferase from eggplant leaves.

81%
Nawłoka P., Kalinowska M., Pączkowski C., Wojciechowski Z. A.
Acta Biochimica Polonica
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2003
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vol. 50
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issue 2
567-572
EN
Effects of several chemical probes selectively modifying various amino-acid residues on the activity of UDP-glucose : solasodine glucosyltransferase from eggplant leaves was studied. It was shown that diethylpyrocarbonate (DEPC), a specific modifier of histidine residues, was strongly inhibitory. However, in the presence of excessive amounts of the enzyme substrates, i.e. either UDP-glucose or solasodine, the inhibitory effect of DEPC was much weaker indicating that histidine (or histidines) are present in the active site of the enzyme. Our results suggest also that unmodified residues of glutamic (or aspartic) acid, lysine, cysteine, tyrosine and tryptophan are necessary for full activity of the enzyme. Reagents modifying serine and arginine residues have no effect on the enzyme activity.
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