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1
Content available remote

Formation of DNA adducts in liver of rats treated with 2-aminofluorene. Differences between total and matrix attached DNA fractions

100%
Widłak P., Rzeszowska-Wolny J.
Acta Biochimica Polonica
|
1990
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vol. 37
|
issue 1
65-67
2
Content available remote

Formation and removal of DNA adducts in liver of rats treated with hepatocarcinogens 2-aminofluorene or 2-acetylaminofluorene

100%
Widłak P., Rzeszowska-Wolny J.
Acta Biochimica Polonica
|
1991
|
vol. 38
|
issue 1
145-150
3
Content available remote

Inhibition of poly(ADP-ribose) polymerase activity affects its subcellular localization and DNA strand break rejoining

81%
Ryabokon N. I., Cieślar-Pobuda A., Rzeszowska-Wolny J.
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|
issue 2
243-248
EN
Poly(ADP-ribose) polymerase (PARP) plays a crucial role in DNA repair. Modulation of its activity by stimulation or inhibition is considered as a potentially important strategy in clinical practice, especially to sensitize tumor cells to chemo- and radiotherapy through inhibition of DNA repair. Here we studied the effect of the three PARP inhibitors, 5-iodo-6-amino-benzopyrone (INH2BP), 1,5-isoquinolinediol (1,5-dihydroxyisoquinolinediol (1,5-IQD) and 8-hydroxy-2-methylquinazolin-4-[3H]one (NU1025), and for two of them the efficiency in slowing the rejoining of DNA strand breaks induced by H2O2 was compared. Inhibition of PARP changed its intranuclear localization markedly; cells exposed to the inhibitor NU1025 showed a significant tendency to accumulate PARP in large foci, whereas in untreated cells its distribution was more uniform. The speed and efficiency of rejoining of H2O2-induced DNA strand breaks were lower in cells incubated with a PARP inhibitor, and the kinetics of rejoining were modulated in a different manner by each inhibitor. At a concentration of 100 µM the efficiency of the inhibitors could be ranked in the order NU1025 > IQD > INH2BP. The two first compounds were able to decrease the overall PARP activity below the level detected in control cells, while INH2BP showed up to 40% PARP activity after exposure to H2O2.
4
Content available remote

Association of SV40 DNA sequences with nuclear matrix in SV40 transformed hamster fibroblasts

81%
Rzeszowska-Wolny J., Belcarz J., Chorąży M.
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|
issue 2
103-110
5
Content available remote

DNA damage and repair in lymphocytes of normal individuals and cancer patients: studies by the comet assay and micronucleus tests.

81%
Palyvoda O., Polańska J., Wygoda A., Rzeszowska-Wolny J.
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2003
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vol. 50
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issue 1
181-190
EN
A population study is reported in which the DNA damage induced by γ-radiation (2 Gy) and the kinetics of the subsequent repair were estimated by the comet and micronucleus assays in isolated lymphocytes of 82 healthy donors and patients with head and neck cancer before radiotherapy. The parameters of background and radiation-induced DNA damage, rate of repair, and residual non-repaired damage were measured by comet assay, and the repair kinetics for every donor were computer-fitted to an exponential curve. The level of background DNA damage before irradiation measured by comet assay as well as the level of micronuclei were significantly higher in the head and neck cancer patient group than in the healthy donors, while the parameters of repair were widely scattered in both groups. Cancer patient group contained significantly more individuals, whose irradiated lymphocytes showed high DNA damage, low repair rate and high non-repaired DNA damage level. Lymphocytes of donors belonging to this subgroup showed significantly lower inhibition of cell cycle after irradiation.
6
Content available remote

Detection of damage-recognition proteins from human lymphocytes.

81%
Łanuszewska J., Cudak A., Rzeszowska-Wolny J., Widłak P.
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2000
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vol. 47
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issue 2
443-450
EN
Proteins recognizing and binding to damaged DNA (DDB-proteins) were analyzed in human lymphocytes obtained from healthy donors. Using an electrophoretic mobility shift assay several complexes between nuclear extract proteins and damaged DNA were detected: a complex specific for DNA damaged by N-acetoxy-N-acetylaminofluorene, another complex specific for UV-irradiated DNA, and two complexes specific for DNA damaged by cis-dichlorodiammine platinum. All the detected complexes differed in electrophoretic mobility and possibly contained different proteins. Complexes specific for free DNA ends were also detected in protein extracts from lymphocytes.
7
Content available remote

Calculation of reliable transcript levels of annotated genes on the basis of multiple probe-sets in Affymetrix microarrays

81%
Jaksik R., Polańska J., Herok R., Rzeszowska-Wolny J.
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issue 2
271-277
EN
Microarray methods have become a basic tool in studies of global gene expression and changes in transcript levels. Affymetrix microarrays from the HGU133 series contain multiple probe-sets complementary to the same gene (4742 genes are represented by more than one probe-set in a microarray HGU133A). Individual probe-sets annotated to the same gene often show different hybridization signals and even opposite trends, which may result from some of them matching transcripts of more than one gene and from the existence of different splice-variant transcripts. Existing methods that redefine probe-sets and develop custom probe-set definitions use mathematical tools such as Matlab or the R statistical environment with the Bioconductor package (Gentleman et al., 2004, Genome Biol. 5: 280) and thus are directed to researchers with a good knowledge of bioinformatics. We propose here a new approach based on the principle that a probe-set which hybridizes to more than one transcript can be recognized because it produces a signal significantly different from others assigned to the particular gene, allowing it to be detected as an outlier in the group and eliminated from subsequent analyses. A simple freeware application has been developed (available at www.bioinformatics.aei.polsl.pl) that detects and removes outlying probe-sets and calculates average signal values for individual genes using the latest annotation database provided by Affymetrix. We illustrate this procedure using microarray data from our experiments aiming to study changes of transcription profile induced by ionizing radiation in human cells.
8
Content available remote

Interactions of the matrix attachment region of DNA with the matrix proteins from the copper preincubated liver nuclei

81%
Widłak P., Rogoliński J., Rzeszowska-Wolny J.
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9
Content available remote

Interactions of rat repetitive sequence MspI8 with nuclear matrix proteins during spermatogenesis

81%
Rogoliński J., Widłak P., Rzeszowska-Wolny J.
Acta Biochimica Polonica
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1996
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vol. 43
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issue 2
319-324
10
Content available remote

DNA and proteins of the nuclear matrix are the main targets of benzo[a]pyrene's action in rat hepatocytes

80%
Widłak P., Rzeszowska-Wolny J.
|
|
vol. 40
|
issue 4
559-562
11
Content available remote

MspI8, the repetitive sequence specifically interacting with nuclear matrix of rat testis cells

80%
Rzeszowska-Wolny J., Rogoliński J.
Acta Biochimica Polonica
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1994
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vol. 41
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issue 4
459-466
12
Content available remote

Damaged DNA-binding proteins: recognition of N-acetoxy-acetylaminofluorene-induced DNA adducts

80%
Rzeszowska-Wolny J., Widłak P.
Acta Biochimica Polonica
|
1999
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vol. 46
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issue 1
173-180
13
Content available remote

Characterization of DNA sequences localized 11 to 17.5 kb upstream of the chicken embryonic π-globin gene

71%
Pietrowska M., Rusin M., Widłak P., Razin S. V., Rzeszowska-Wolny J.
Acta Biochimica Polonica
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1999
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vol. 46
|
issue 3
777-784
14
Content available remote

Metrizamide gradient centrifugation of histone-DNA complexes

71%
Rzeszowska-Wolny J., Gröbner S., Filipski J., Chorąży M.
Acta Biochimica Polonica
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1982
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vol. 29
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issue 3-4
289-298
15
Content available remote

Effects of the hepatocarcinogen 2-aminofluorene on RNA synthesis in rat liver

70%
Widłak P., Rzeszowska-Wolny J.
Acta Biochimica Polonica
|
1992
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vol. 39
|
issue 1
21-26
16
Content available remote

Interaction of DNA with nuclear skeleton in rat hepatocytes

61%
Widłak P., Rogoliński J., Rzeszowska-Wolny J.
Acta Biochimica Polonica
|
1990
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vol. 37
|
issue 1
69-72
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