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Biofilm formation and serum susceptibility in Pseudomonas aeruginosa

100%

Mikucionyte G., Dambrauskiene A., Skrodeniene E., Vitkauskiene A.

Open Medicine

2014

vol. 9

issue 2

187-192

Pseudomonas aeruginosa (P. aeruginosa) is one of the most important opportunistic pathogens. The pathogenicity of P. aeruginosa has been associated with multiple bacterial virulence factors. The aim of this study was to evaluate the association between P. aeruginosa strains obtained from various clinical samples and resistance to antibiotics and pathogenicity factors, such as resistance to serum bactericidal activity and biofilm formation. This study included 121 P. aeruginosa strains isolated from clinical samples; 65 of the isolated P. aeruginosa strains were carbapenem-resistant, and 56 were carbapenem-sensitive. Carbapenem-resistant P. aeruginosa strains were more often resistant to the majority of tested antibiotics, compared to carbapenem-sensitive strains. We did not find any statistically significant difference between resistance to carbapenems and serum resistance and ability of tested P. aeruginosa strains to produce biofilms. Carbapenem-resistant P. aeruginosa strains were recovered from the urinary tract significantly more often (75.0%) than carbapenem-sensitive P. aeruginosa strains (25.0%). Carbapenem-sensitive P. aeruginosa strains were recovered significantly more often from the respiratory tract than carbapenem-resistant strains, 60.0% and 40.0%, respectively. All the P. aeruginosa strains recovered from blood were serum-resistant. P. aeruginosa strains recovered from the respiratory tract and wounds were significantly frequently serum sensitive, 95.6% and 56.6%, respectively. We did not find any differences in biofilm production among the P. aeruginosa strains recovered from different sources.

Cytokine production by leukocytes in patients with periodontitis

76%

Sakalauskiene J., Giedrimiene D., Kubilius R., Gleiznys A., Vitkauskiene A., Ivanauskiene E., Puskunigyte K.

Open Medicine

2014

vol. 9

issue 6

821-829

In the present study, we investigated the relation between clinical parameters and levels of interleukin (IL) -4 and -5, and tumour necrosis factor-α (TNF-α) in the leukocyte incubation medium (LIM) obtained from 26 patients with chronic periodontitis (P) and 26 control group subjects (C). The levels of cytokines IL-4 and IL -5 produced by the LIM stimulated with non-opsonised E. coli were determined using the Enzyme-Linked Immunosorbent Assay (ELISA) method and the levels of TNF-α were evaluated by applying Enzyme Amplified Sensitivity Immunoassay (EASIA). TNF-α levels in stimulated LIM were strongly positively correlated with clinical parameters such as the pocket probing depths (PPD), the clinical attachment level (CAL), the bleeding on probing (BOP) and oral hygiene index (OHI), whereas the IL-4 and IL-5 levels in the analogous medium were strongly negatively correlated with the clinical parameters. IL-4 and IL-5 levels in stimulated LIM of P group patients were significantly lower, whereas TNF-α levels were significantly higher than that in analogous medium of C group subjects. These differences were associated with the severity of periodontal disease.

Effects of β-1.3/1.6 glucan on cytokines production by leukocytes in vitro

76%

Sakalauskiene J., Akramas L., Gleiznys A., Kubilius R., Vitkauskiene A., Surna A., Junevicius J.

Open Medicine

2012

vol. 7

issue 6

800-807

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3 Open Medicine

2 2014

1 2012

3 Vitkauskiene A.

2 Gleiznys A.

2 Kubilius R.

2 Sakalauskiene J.

1 Akramas L.

1 Dambrauskiene A.

1 Giedrimiene D.

1 Ivanauskiene E.

1 Junevicius J.

1 Mikucionyte G.

1 Puskunigyte K.

1 Skrodeniene E.

1 Surna A.

Search results

Biofilm formation and serum susceptibility in Pseudomonas aeruginosa

Cytokine production by leukocytes in patients with periodontitis

Effects of β-1.3/1.6 glucan on cytokines production by leukocytes in vitro