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Total Phenolic, Flavonoid Contents and Antioxidant Activities of Honey and Propolis Collected from the Region of Laghouat (South of Algeria)

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Bakchiche B., Habati M., Benmebarek A., Gherib A.

World News of Natural Sciences

2017

vol. 11

91-97

The aim of this study was to determine total phenolic and flavonoid contents and to evaluate the antioxidant activities of two honeys and one propolis sample, collected from the region of Laghouat (South of Algeria). Total phenolic content were determined by using Folin-Ciocalteu reagent as gallic acid equivalent, while flavonoids content was assessed by means of the AlCl3 method as Rutin equivalent. Antioxidant activities of the honeys and propolis were examined by two different methods, namely, scavenging of free radical 2,2-diphenyl-1-picrylhydrazyl and reducing power. The antioxidant activities were compared with standard antioxidants such as Ascorbic acid, BHT and Trolox. The highest level of phenolic was 2385 mg Gallic acid per 100g sample, the highest level of flavonoid was 379 mg Rutin per 100g sample, while the highest protein content was 1177 mg per 100g sample, DPPH (0.026 mg/ml) and TEAC (0.0015) were detected especially in the propolis sample, indicating that it holds good antioxidant properties. A strong positive correlation was found between phenolics, flavonoids, DPPH and TEAC, indicating that in addition to total phenolic content, flavonoid and protein concentrations are good indicators of the antioxidant potential of propolis and honey.

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1 World News of Natural Sciences

1 2017

1 Bakchiche B.

1 Benmebarek A.

1 Gherib A.

1 Habati M.

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Total Phenolic, Flavonoid Contents and Antioxidant Activities of Honey and Propolis Collected from the Region of Laghouat (South of Algeria)