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Technological and nutritious aspects of enzymatic enrichment of milk and it's derivatives in galactooligosaccharides

100%

Demczuk A., Bednarski W., Kowalewska-Piontas J.

Biotechnologia

2004

issue 3

152-166

Enzymatic hydrolysis of lactose is accompanied by transgalactosylation reaction, thereby producing oligosaccharides. The prebiotic functions of oligosaccharides such as bifidogenic effect and health benefits connected with this effect were described. The mechanism of galactooligosaccharides formation and factors affecting it were also presented. Moreover, the examples of an enzymatic enrichement of milk and it's derivatives in galactooligosaccharides were given.

Trials of stimulation extracellular secretion of beta-galactosidase synthesised by Kluyveromyces fragilis 28

100%

Demczuk A., Kowalewska-Piontas J., Bednarski W.

Biotechnologia

2004

issue 1

253-259

To induce extracellular secretion of beta-galactosidase synthesised by Kluyveromyces fragilis 28 yeasts, we used: glycin, L-asparagine, L-leucine, dimethylformamide, dimethyl sulfoxide, cetyldimethylethylammonium bromide, penicillin G and glycolipids from Candida antarctica. The highest increase in the secretion of beta-galactosidase was obtained in the yeast culture cultivated in the medium with polypeptone when glycin was used as the secretion inductor. The extracellular activity of beta-galactosidase reached 0.416 A.U./ml, and was 10-fold higher than the beta-galactosidase activity reported in the control group.

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2 Biotechnologia

2 2004

2 Bednarski W.

2 Demczuk A.

2 Kowalewska-Piontas J.

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Technological and nutritious aspects of enzymatic enrichment of milk and it's derivatives in galactooligosaccharides

Trials of stimulation extracellular secretion of beta-galactosidase synthesised by Kluyveromyces fragilis 28