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1

Resistance to antibiotics of the selected species of Agrobacterium rhizogenes

100%
Krolicka A., Kurlenda J., Lojkowska E.
Biotechnologia
|
1999
|
issue 2
94-103
EN
Agrobacterium rhizogenes is used for the transformation of plant cells and production of hairy roots cultures. In the presented work the bacteriostatic activity of several antibiotics on A. rhizogenes strains was tested. Different concentration of antibiotics belonging to the types of cefalosporin II and III generation, b-lactam and fluorochinolon were tested for elimination of the bacteria from transformed tissue. Out of all tested combinations, the mixture of carbenicilin and cefotaksym (claforan) was the most efficient for A. rhizogenes strains elimination from transformed plant tissues. The addition of those antibiotics to the regeneration medium was not toxic to plant tissues and it facilitated rapid growth of hairy roots.
2

Thiol?activated hemolysins as virulence markers of bacteria

88%
Szramka B., Kurlenda K. J., Kurlenda J., Podhajska P. A. J., Podhajska A. J.
Biotechnologia
|
2001
|
issue 3
152-168
EN
The article contains information regarding the similarities and differences between proteins of the thiol-activated cytolysins family (TACY). Members of this group of haemolysins are produced by several species of Gram-positive bacteria (Listeria spp., Streptococcus spp., Bacillus spp., Clostridium spp.,) and also by Gram-negative Klebsiella spp.. Their cytolytic activity is sensitive to oxygen and can be restored by adding reducing compounds. TACY bind to cholesterol-containing membranes to form pores. Preincubation of the toxin with small amounts of cholesterol inhibits hemolytic activity as well as cytolysis of eucaryotic cells. Members of the group show 40-70% similarity in amino acid sequence, and contain an almost invariant Trp-rich undecapeptide sequence (ECTGLAWEWWR). The TACY are important virulence factors. Recently increased use has been made of molecular methods (PCR, hybridization) in the detection and identification of the TACY producing bacteria.
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