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1

Obtainng of hybrids within the family Cucubitaceae by in vitro culture of immature embryos.III.Characteristics of hybrids of Cucurbita maxima x C.ficifolia and C.maxima x C.foetidissima

100%
Plader W., Rakoczy-Trojanowska M.
Genetica Polonica
|
1994
|
vol. 35
|
issue 1-2
11-22
2

Identification of transcriptionally active regions with genome tiling microarrays

81%
Zmienko A., Guzowska-Nowowiejska M., Plader W., Figlerowicz M.
Biotechnologia
|
2008
|
issue 4
101-114
EN
Genome tiling microarrays, covering whole genomic sequence, have gained increasing popularity in transcript mapping studies. Functional analysis of model eucaryotic and procaryotic genomes proved their sensitivity and versatility in discovering actively transcribed regions of the genomes. Many novel proteins coding genes, miRNA coding genes, antisense transcripts and other non-protein coding regulatory RNAs, transcribed from introns, intergenic and centromeric regions have been identified this way. Their expression can often be linked to specific developmental stages, organs or stress response in plants and animals, giving further insight into processes which were considered to be already well characterized.
3

Transgene inheritance in plants

81%
Yin Z., Plader W., Malepszy S.
Journal of Applied Genetics
|
2004
|
vol. 45
|
issue 2
127-144
EN
The patterns of transgene inheritance in plants and the possible explanations for non-Mendelian transmission are reviewed. The non-Mendelian inheritance of a transgene has been recorded with a frequency between 10% and 50% in transgenic plants produced either by Agrobacterium-mediated transformation or through particle bombardment. Different effects such as deletion, duplication, rearrangement, repeated sequence recombination as well as gene interaction have been observed for transgenic loci. The nature of the recipient genome, nature of the transgene and the interactions between them seem to contribute to the non-Mendelian segregation of transgenes.
4

Sequential fluorescent staining with CMA and DAPI for somatic chromosome identification in cucumber (Cucumis sativus L.)

81%
Plader W., Hoshi Y., Malepszy S.
Journal of Applied Genetics
|
1998
|
vol. 39
|
issue 3
249-258
EN
Each of the seven chromosomes in cucumber (Cucumis sativus L.) was identified using sequential staining with Chromomycin A3 (CMA) and 4-6-diamidino-2-phenylindole (DAPI) as DNA base-specific fluorescent dyes. The present method using enzymatic digestion produced a high level of well-spread early-metaphase chromosome complements. After CMA and DAPI staining, reproducible fluorescence bands were obtained in mitotic prometaphase chromosomes. The CMA staining method made it possible to characterize whole chromosomes from prometaphase to mid-metaphase. Chromosome 1 had the largest and widest CMA-positive (CMA+) band from the proximal region to the interstitial region on the long arm in prometaphase. A large gap separating of the short arm from the long arm was always observed in chromosome 2 during prometaphase. The banding pattern of the short arm was similar to that of the long arm in chromosome 2. Chromosomes 1 and 2 in early metaphase had sharp and large CMA-positive and DAPI-negative (CMA+DAPI-) bands at the pericentromeric regions. In early metaphase, chromosome 3 was characterized by having a narrow CMA+DAPI- band on the pericentromeric region of the short arm. Chromosomes 4 and 5 showed similar chromosome length and had a large CMA+ band at the distal region of the long arm. Chromosome 4 did not show any clear band in the short arm, while chromosome 5 showed a telomeric CMA+ band at the short arm and a clear CMA+DAPI- band at the pericentromeric region. Chromosome 6 had a CMA+ band at the distal region and a weak CMA+ band at the proximal region in each of the arms. Chromosome 7 had an evident CMA+ band in the long arm and a CMA+DAPI- band in the pericentromeric region.
5

Chromosome number variation in somatic hybrids between transgenic tomato (Lycopersicon esculentum) and Solanum lycopersicoides

61%
Kulawiec M., Tagashira N., Plader W., Bartoszewski G., Kuc D., Sniezko R., Malepszy S.
Journal of Applied Genetics
|
2003
|
vol. 44
|
issue 4
431-447
EN
Leaf mesophyll protoplasts of Lycopersicon esculentum were fused with suspension-culture-derived protoplasts of Solanum lycopersicoides by a PEG treatment. Both species have the same chromosome number (2n = 2x = 24). The hybrid calli were selected using the full selection method ? kanamycin resistance and culture conditions critical for L. esculentum protoplast divisions. The genomic in situ hybridization analyses indicated a hypo- and hypertetraploid character of the hybrid plant with a majority of S. lycopersicoides chromosomes and a variation in chromosome number from 46 to 53. The hybrids contained a transgene derived from L. esculentum, as shown by Southern blot hybridization and PCR analyses. Their mitochondria were derived from the wild species, S. lycopersicoides. More than 60 regenerated plants were transferred into the greenhouse. They grew very slowly and were not able to flower for almost one year. The main morphological characters of the hybrids included a single shoot and small, dark-green leaves with strongly wrinkled blades. The reasons for nuclear genome asymmetry between hybrids and the possibilities of using them in a genetic and breeding programme are discussed in this paper.
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