Laccases catalyze oxidative conversion of a broad range of substrates such as phenols or lignin-derivatives. They also have an ability to induce oxidative coupling reactions of the free radical intermediates to related phenolic structures. The application of laccases-mediated coupling reactions in white biotechnology represents a promising alternative to chemical synthesis of products of commercial importance. The goal of this review was to present potential application of fungal laccases, focusing specifically on their use as biocatalysts in environmental friendly synthesis of new molecules.
The biodegradation of dyes in textile wastewater is nowadays a subject of intensive research. In particular, a biotransformation of various dyestuffs by white-rot fungi is the most promising alternative. This study provides the results of decolorization of the aqueous solution of azo- and anthraquinone dyes by the laccase complex from Cerrena unicolor. The investigations were carried out using 3 systems: with active biomass, with sterilized biomass (biosorption), and with secretion of enzymatic complex. The enzymatic biotransformation gave to 97% decolorization of Acid Blue 62 and 95% decolorization of Reactive Blue 81 after 15 min. The dye?s adsorption on mycelia contributes substantially (30-40%) to decolorization of the textile wastewater.
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