Full-text resources of PSJD and other databases are now available in the new Library of Science.
Visit https://bibliotekanauki.pl
Preferences help
Polish version English version Language
enabled [disable] Abstract
Number of results

Results found: 3

Number of results on page
first rewind previous Page / 1 next fast forward last

Search results

help Sort By:

help Limit search:
first rewind previous Page / 1 next fast forward last
1

The oviduct of transgenic birds ? a source of therapeutic proteins?

100%
Bednarczyk M., Lakota P., Wawrzynska M.
Biotechnologia
|
2006
|
issue 3
134-144
EN
The use of transgenic mammals for the production of therapeutic proteins was developed over two decades ago, and a number of proteins have been produced in the milk of animals. Therapeutic proteins produced in eggs of transgenic hens have significant advantages over transgenic mammalian systems, including: regular high eggs production, large number of progeny, short generation times, sterile contents of the eggs produced by specific pathogen free chickens and, well characterization of the regulatory sequences of white protein genes similar to human glycosylation of chicken IgGs, etc. In this overview, recent progress in the development of chicken bioreaktors was presented.
2

Influence of electroporation on chicken blastoderm cell viability in vitro

100%
Wawrzynska M., Bednarczyk M., Lakota P., Lubiszewska M.
Folia Biologica
|
2008
|
vol. 56
|
issue 3-4
197-201
EN
The aim of this study was to compare two types of devices used for blastoderm cell (BC) transfection: the Nucleofector (Amaxa, Biosystems) and the Multiporator (Eppendorf). To assess the influence of electric current on BCs, different conditions of both nucleofection and electroporation were used. Next, the viability of cells was assessed. The highest number of cells (90.8%) was viable after nucleofection in the G10 program,. After transfection in the presence of pmaxGFP, the A23 program was found to be most advantageous. The elecroporation experiment with theMultiporator (Eppendorf) showed a significant influence of osmotic pressure and voltage on BC viability. Namely, in the isoosmolar buffer BC viability was statistically higher (P#0.05) in comparison to the hypoosmolar buffer. The, viability of cells was statistically higher (P#0.05) after application of 25V as compared to 50V. The efficiency of transfection in the presence of EGFP-C1 after electroporation in 2 pulses, 25V, 500 ?s in the isoosmolar buffer was better than in the recommended conditions in the Amaxa Biosystems A23 program.
3

Expression of exogenous genes in blastodermal cells of chicken in vitro

76%
Bednarczyk M., Plucienniczak G., Plucienniczak A., Lakota P., Sochanik A., Dluzniewska A., Grajewski B.
Folia Biologica
|
2003
|
vol. 51
|
issue 3-4
189-194
EN
Chicken blastodermal cells (BCs) from stage X embryos produce both somatic and germline chimeras when injected into the subgerminal cavity of recipient embryos. Transfection of the donor cells in vitro could lead to the production of chimeras capable of transmitting the transgene to their offspring. The aim of this study was to transfer and express foreign genes under control of the ovalbumin promoter in the BCs. The results showed that luciferase activity in the BCs reached a plateau value with a 2.0:1.0 or 5.0:1.0 liposome ? DNA ratio and using 1mug of DNA. Under this same condition, no difference was found in relative activity between the pGL-control and pOVALUC plasmid. The expression of other exogenous genes (green fluorescent protein and interferon alpha2a) driven by the chicken ovalbumin promoter in cultured chicken blastodermal cells in vitro is possible by this assay. Hatchability of recipient embryos after injection of 1, 500 or 800 transfected BCs was compared. The advantage of using a smaller number (800) of injected transfected BCs was that early embryonic mortality was reduced and resulted in higher (P<0.01) hatchability (24.5%) than in the case of 1, 500 BCs injected.
first rewind previous Page / 1 next fast forward last
JavaScript is turned off in your web browser. Turn it on to take full advantage of this site, then refresh the page.