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NEW AFFINITY SORBENTS FOR PURIFICATION OF RECOMBINANT PROTEINS WITH THE USE OF CHITIN-BINDING DOMAIN AS AN AFFINITY TAG

100%
Kurek D. V., Lopatin S. A., Tikhonov, V. E., Varlamov V. P.
Progress on Chemistry and Application of Chitin and its Derivatives
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2010
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vol. 15
41 - 46
EN
With the increasing amounts of recombinant proteins production the problem of effective purification of produced proteins became one of major topics for research nowadays. Affinity chromatography proved to be the most dominant method for this aim and application of chitin based affinity sorbents in combination with use of chitin-binding domain as an affinity tag for purification of recombinant proteins showed its efficiency even in comparison with commercially available systems.
2
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MOLECULAR STRUCTURE AND FORMATION OF CHITOSAN AND PECTIN BASED THIN FILMS

100%
Chudinova Y. V., Kurek D. V., Varlamov V. P.
Progress on Chemistry and Application of Chitin and its Derivatives
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2016
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vol. 21
18-26
EN
In this study using methods of Atomic force microscopy (AFM) and Quartz crystal microbalance with dissipation monitoring (QCM-D) the special characteristics of formation and architecture of thin films coatings based on natural polysaccharides chitosan and pectin were investigated. A layer-by-layer (LbL) deposition technique for assembling of oppositely charged layers was used. The main factors, which have an influence on the process of thin film formation and molecular structure of these coatings, were investigated.
3
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PREPARATION AND CHARACTERIZATION OF CRYOGELS BASED ON PECTIN AND CHITOSAN

88%
Konovalova M. V., Kurek D. V., Litvinets S. G., Martinson E. A., Varlamov V. P.
Progress on Chemistry and Application of Chitin and its Derivatives
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2016
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vol. 21
114-121
EN
Novel cryogels based on pectin and chitosan were obtained by a cryotropic gelation method. Percentage of chitosan in the cryogels was estimated by elemental analysis. The obtained values were from 3.50 to 15.03 % for Apple pectin/Chitosan cryogels and from 9.44 to 17.64 % for Heracleum pectin/Chitosan cryogels. Internal structure and porosity of the cryogels were measured by scanning electron microscopy. According to the scanning electron microscopy, cryogels have a macroporous sheet-like structure. In the future, cryogels can be used in biotechnology and medicine as biocompatible, biodegradable materials.
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