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1

Variability of seed protein composition of hulless mutants of spring barley (Hordeum vulgare L.)

100%
Kapala A., Rybinski W.
Journal of Applied Genetics
|
1995
|
vol. 36
|
issue 2
129-135
EN
Nutritional value of protein in 146 mutants of hulles spring barley was estimated.Evaluation of protein quality was based on the portion of a relative content of protein fractions with different amino acid composition and, therefore, with different nutritional value.The ratio of albumins, globulins and gluteins to hordein, determing nutritional value of protein in the initial genotype was 1.1; in mutants it was within the range of 0.9-1.5. Several mutants with a high protein content and good nutritional value have been distinguished.
2

Genetic variation of hordein polypeptides in grains of mutants of hulles spring barley (Hordeum vulgare L.) breeding line

100%
Kapala A., Rybinski W.
Journal of Applied Genetics
|
1996
|
vol. 37
|
issue 1
29-35
EN
Hordein polypeptides pattern of 146 mutants in M7 from a hulless spring barley breeding line were examined.The mutants were obtained after treatment of grains of Polish hulless spring barley line, 1N/86, with the chemomutagens N-nirtiso-N-methylurea and sodium azide.Tehmutants were distinguished from the parential line with regard to morphological and yield structure traits.SDS-PAGE of the main hordein fractons, B and C, showed thar the elecrophoretic patterns of the majority of the analysed mutants were similar to the hordein polypeptide spectrum of the parental line.In the previous study, the same mutants showed a significantly better feeding value as compared to hulled cultivars and the rest of the analysed mutants.
3

The use of laser light for obtaining barley haploids by Hordeum bulbosum method

81%
Rybinski W., Surma M., Adamski T.
Biotechnologia
|
2001
|
issue 1
143-147
EN
The effect of helium-neon laser with wavelength of 632 nm on seed setting in a cross of Hordeum vulgare x H. bulbosum was studied. The seeds before sowing as well as immature embryos were irradiated with laser light. Material not irradiated with laser beams constituted the control. It was shown that stimulative dose of laser beams increased the number of seeds/100 pollinated florets in comparison with the control combination. After laser treatment, the seed setting of the line HG156, and cultivars Vada and Apex was higher by 13.0, 7.9 and 3.2%, respectively. The number of obtained haploids/100 cultured embryos irradiated with laser light was also higher in comparison with the control by 1.8, 0.9 and 10.8%, respectively. The obtained results show that treatment with laser beams was more effective at the first step of haploid production (seeds/100 florets) than at the next step (haploids/100 embryos cultured). From the practical point of view, better results can be obtained by irradiation of seeds before sowing than by irradiation of immature embryos. Cultivar Apex with a positive reaction on irradiation of embryos was an exception here.
4

Mutagenic effect of laser and chemical mutagens in barley (Hordeum vulgare L.)

81%
Rybinski W., Patyna H., Przewozny T.
Genetica Polonica
|
1993
|
vol. 34
|
issue 4
337-342
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