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Ribozymes of the hepatitis delta virus: Recent findings on their structure, mechanism of catalysis and possible applications.

100%

Ciesiołka J., Wrzesinski J., Łęgiewicz M., Smólska B., Dutkiewicz M.

2001

vol. 48

issue 2

409-418

Although the delta ribozymes have been studied for more than ten years the most important information concerning their structure and mechanism of catalysis were only obtained very recently. The crystal structure of the genomic delta ribozyme turns out to be an excellent example of the extraordinary properties of RNA molecules to fold into uniquely compact structures. Details of the X-ray structure have greatly stimulated further studies on the folding of the ribozymes into functionally active molecules as well as on the mechanism of RNA catalysis. The ability of the delta ribozymes to carry out general acid-base catalysis by nucleotide side chains has been assumed in two proposed mechanisms of self-cleavage. Recently, considerable progress has been also made in characterizing the catalytic properties of trans-acting ribozyme variants that are potentially attractive tools in the strategy of directed RNA degradation.

Translational and structural analysis of the shortest legume ENOD40 gene in Lupinus luteus

59%

Podkowinski J., Zmienko A., Florek B., Wojciechowski P., Rybarczyk A., Wrzesinski J., Ciesiolka J., Blazewicz J., Kondorosi A., Crespi M., Legocki A.

issue 1

89-102

Two early nodulin 40 (enod40) genes, ENOD40-1, the shortest legume ENOD40 gene, and ENOD40-2, were isolated from Lupinus luteus, a legume with indeterminate nodules. Both genes were expressed at similar levels during symbiosis with nitrogen-fixing bacteria. ENOD40 phylogeny clustered the L. luteus genes with legumes forming determinate nodules and revealed peptide similarities. The ENOD40-1 small ORF A fused to a reporter gene was efficiently expressed in plant cells, indicating that the start codon is recognized for translation. The ENOD40-1 RNA structure predicted based on Pb(II)-induced cleavage and modeling revealed four structurally conserved domains, an absence of domain 4 characteristic for legumes of indeterminate nodules, and interactions between the conserved region I and a region located upstream of domain 6. Domain 2 contains Mg(II) ion binding sites essential for organizing RNA secondary structure. The differences between L. luteus and Glycine max ENOD40 RNA models suggest the possibility of a switch between two structural states of ENOD40 transcript.

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2 Acta Biochimica Polonica

1 2009

1 2001

2 Wrzesinski J.

1 Blazewicz J.

1 Ciesiolka J.

1 Ciesiołka J.

1 Crespi M.

1 Dutkiewicz M.

1 Florek B.

1 Kondorosi A.

1 Legocki A.

1 Podkowinski J.

1 Rybarczyk A.

1 Smólska B.

1 Wojciechowski P.

1 Zmienko A.

1 Łęgiewicz M.

Search results

Ribozymes of the hepatitis delta virus: Recent findings on their structure, mechanism of catalysis and possible applications.

Translational and structural analysis of the shortest legume ENOD40 gene in Lupinus luteus