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Proximate composition, antioxidant activities and fatty acid profiling of selected Mushrooms collected from Azad Jammu and Kashmir

100%

Shahid M., Fatima H., Anjum F., Riaz M.

Acta Poloniae Pharmaceutica - Drug Research

2020

vol. 77

issue 1

145-153

Mushrooms can be used in diet as nutraceutical or functional foods for promoting and maintaining health quality and health longevity. Mushrooms just like medicinal plants can be utilized in the form of powder or extract as curative agents of several diseases and in providing a healthy diet. In current study, ten different mushroom species were collected from Azad Jammu and Kashmir. The selected mushrooms were assessed for their nutritional value, biological activities and fatty acid profiling. Before extraction, proximate analysis of all selected mushrooms was performed. Protein, fat, ash and total carbohydrate contents ranged from 10-30%, 0.43-8.08%, 1.1-9.14%, 12.47-92.52%, respectively. Then mushrooms were extracted with three different solvents n-hexane, ethyl acetate and methanol for the assessment of their antioxidant potentials through different antioxidant assays like total phenolics, total flavonoid contents and DPPH inhibition assay. The order of antioxidant potential in different solvent is methanol>Ethyl acetate>n-hexane. Quantification of fatty acids by GC/MS analysis revealed that stearic acid, palmitic acid, oleic acid, linolenic acid were present in significant quantity in different mushrooms and unsaturated fatty acids were found abundant over saturated fatty acids in all the selected mushrooms. So presence of fatty acids in studied samples is one of the main biologically active ingredients from mushrooms. The study concluded that the selected mushrooms from different regions of Azad Jammu and Kashmir are of nutritional importance having significant antioxidant potential and contains several unsaturated fatty acids which contribute to the nutritional value of mushrooms.

Triggering of erythrocyte membrane blebbing by ciprofloxacin

76%

Shabir K., Jilani K., Zbidah M., Riaz M., Bibi I., Asghar A.

Acta Poloniae Pharmaceutica - Drug Research

2019

vol. 76

issue 5

901-906

An extensively used fluoroquinolone antibiotic ciprofloxacin shows a broad-spectrum antibacterial activity against both gram-positive and gram-negative strains. It works mainly by the inhibition of DNA gyrase and topoisomerase IV which results in impaired DNA replication leading towards microbial cell death. Eryptosis is an alternative term used for suicidal erythrocyte death. In current study, eryptotic effect of ciprofloxacin was investigated by exposing erythrocytes for 48 hours to the different concentrations (45-90µM) of ciprofloxacin. The experimental work related to the investigation of eryptosis was done by cell size measurement and confirmation of calcium role in membrane blebbing. As a possible mechanism of eryptosis, oxidative stress induced by ciprofloxacin was determined by catalase, glutathion peroxidase and superoxide dismutase activities measurement. Similarly, necrotic effect of ciprofloxacin was also illustrated by hemolysis measurement. Results of our study revealed that the therapeutical doses of ciprofloxacin may induce oxidative stress by significant decrease in superoxide dismutase, catalase and glutathione peroxidase activities as well as induce eryptosis, featured by erythrocytes membrane blebbing and hemolysis.

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2 Acta Poloniae Pharmaceutica - Drug Research

1 2020

1 2019

2 Riaz M.

1 Anjum F.

1 Asghar A.

1 Bibi I.

1 Fatima H.

1 Jilani K.

1 Shabir K.

1 Shahid M.

1 Zbidah M.

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Proximate composition, antioxidant activities and fatty acid profiling of selected Mushrooms collected from Azad Jammu and Kashmir

Triggering of erythrocyte membrane blebbing by ciprofloxacin