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Microorganism Associated with Cassava Fermentation for Abacha Product Sold in Owerri, Imo State

100%

Ihenetu F. C., Ihenetu S. C., Ohalete C. N., Njoku-Obi T. N., Okereke B. C.

World News of Natural Sciences

2017

vol. 12

43-50

The microorganism associated with cassava fermentation for producing Abacha was investigated using four different Farms in Owerri (Obinze, Umugwuma, Urata and Orji) as sample collection areas. Four of the cassava tubers that harvested, were washed, cooked and soaked with water after cutting to produce Abacha, which was then fermented using standard microbiological methods. The wastewater was subsequently sampled. All the samples A, B, C, and D showed significant bacteria growth after application of NA, MRS, PDA. In the study, six bacteria and fungi isolates were identified: Escherichia coli, Staphylococcus aureus, Lactobacillus spp, Candida spp., Aspergillus spp. and Bacillus spp. Results showed that the total heterotrophic bacteria count of the sample varies within 8.0×1010 to 2.5×1012 and total fungi count (TFC) ranges from 6.6×109 to 9.6×109. The study also showed that Lactobacillus spp. and Escherichia coli had the highest prevalence occurrence of 75.00% (P < 0.05), while Staphylococcus aureus, Saccharomyces spp., Bacillus spp. and Candida spp. had a percentage occurrence of 50.00%, respectively. There is, therefore, urgent need for public enlightenment on public health implications, need for proper hygiene, as well as need for strategies for preventing and controlling the microorganisms which do not play a role in the fermentation of cassava for Abacha production.

G6PD Activity in Malaria Infected Children in Owerri

73%

Ihenetu F. C., Nwachukwu M. I., Ohalete C. N., Njoku-Obi T. N., Nwachukwu O. I., Okoro C. I., Okereke B. C., Ihenetu S. C.

World News of Natural Sciences

2017

vol. 12

63-72

This paper is a study of G6PD activity in malaria-infected children in Owerri. Blood samples were collected from fifty-one (51) children hospitalized in the Federal Medical Center (FMC) and analyzed using standard Medical Laboratory methods. Results revealed that out of the 51 children examined (26 males and 25 females), 39, representing 76.47% of the total malaria infected children numbers, were G6PD deficient, while 12, representing 23.53%, had normal G6PD. Two different plasmodium species and their percentage occurrences were observed. These were: Plasmodium falciparum (78.43%) and Plasmodium malariae (21.57%). The work indicated that male children were more (P < 0.05) deficient than females, with percentage levels of 61.53% and 38.47%, respectively. In addition, children between the ages of 49-60 months was observed to be more (P < 0.05) G6PD deficient (with percentage of 25.64%), while those between 0-12 months were least G6PD deficient (with percentage of 8.3% (P < 0.05)). As being afflicted with malaria carries a high risk of Glucose-6-phosphate dehydrogenase (G6PD) deficiency in children, there is, therefore, urgent need for public enlightenment on public health implications, need for proper hygiene, as well as a need for strategies for preventing and controlling mosquito populations

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2 World News of Natural Sciences

2 2017

2 Ihenetu F. C.

2 Ihenetu S. C.

2 Njoku-Obi T. N.

2 Ohalete C. N.

2 Okereke B. C.

1 Nwachukwu M. I.

1 Nwachukwu O. I.

1 Okoro C. I.

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Microorganism Associated with Cassava Fermentation for Abacha Product Sold in Owerri, Imo State

G6PD Activity in Malaria Infected Children in Owerri