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Implementation of NSOM to Biological Samples

100%
Prauzner-Bechcicki S., Wiltowska-Zuber J., Budkowski A., Lekka M., Rysz J.
Acta Physica Polonica A
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2012
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vol. 121
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issue 2
533-538
EN
Near-field scanning optical microscopy is a technique providing images of structures with spatial resolution better than λ/2, which is undetectable in far-field where the Abbe law of limiting resolution is critical. In parallel to the optical imaging, topography maps are also acquired. Near-field scanning optical microscopy measurements can be performed both in air and liquid environments. The later makes the technique very useful for biomaterials analysis offering information that could not be obtained with other methods. Our work presents the results of recent studies on application of near-field scanning optical microscopy to imaging of cells in air as well as in physiological buffers. Differences in cell's topography and morphology have been noticed between two cell lines from human bladder non-malignant (HCV29) and malignant (T24) cancers. Presented results are part of the research that characterizes physiological changes of cells depending on stage of cancer.
2

TNF-alpha priming effect on polymorphonuclear leukocyte reactive oxygen species generation and adhesion molecule expression in hemodialyzed patients

86%
Rysz J., Banach M., Stolarek R. A., Pasnik J., Cia?kowska-Rysz A., Markuszewski L., Baj Z.
Archivum Immunologiae et Therapiae Experimentalis
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2006
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vol. 54
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issue 3
209-215
EN
Introduction: The study aimed to assess reactive oxygen species generation and the expressions of some surface antigens on polymorphonuclear leukocytes (PMNs) in patients on regular hemodialysis (HD) treatment. Materials and Methods: The respiratory burst of PMNs was determined with luminol-dependent chemiluminescence (CL) in resting cells and following N-formyl-methionyl-leucyl-phenylalanine (fMLP), phorbol 12-myristate 13-acetate (PMA), or opsonized zymosan (OZ) stimulation and expressed in arbitrary CL units times assay-time (aUmin). The expressions of CD11b/CD18, CD10, and CD13 receptors were determined with flow cytometry. Results: Basal PMN CL was increased in HD patients to up to 1285129 aUmin compared with 89588 aUmin in healthy controls (p<0.05). The CL of unprimed PMNs increased after fMLP stimulation from 3085746 to 4529808 aUmin, and after OZ stimulation from 129451296 to 146781355 aUmin. PMA-stimulated CL of PMNs was similar to control values. The oxidative burst in PMNs from HD patients and healthy controls was similar in response to TNF-alpha alone. The CL of TNF- alpha-primed PMNs in HD patients was significantly lower than CL measured in healthy controls (p<0.05). The expressions of CD10 and CD13 metalloproteinase receptors were also increased (p<0.05). Although CD11b expression was significantly increased at rest and after fMLP stimulation, the expression of another beta-integrin heterodimer compound, CD18, was not increased. Conclusions: These results provide evidence that TNF- priming of PMNs is down-regulated in HD patients despite constitutive up-regulation of resting cytotoxicity and enhanced expression of adhesion and metalloproteinase receptors.
3

Increased whole blood chemiluminescence in patients with chronic renal failure independent of hemodialysis treatment

73%
Rysz J., Potargowicz E., Banach M., Luczynska M., Stolarek R., Bialasiewicz P., Kasielski M., Cialkowska-Rysz A., Nowak D.
Archivum Immunologiae et Therapiae Experimentalis
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2006
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vol. 54
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issue 5
347-352
EN
Introduction: The luminol-enhanced whole blood chemiluminescence (LBCL) assay is a rapid assay for the measurement of reactive oxygen species (ROS) generation by circulating phagocytes. This study's aim was to determine if patients on maintenance hemodialysis (HD) and non-dialyzed patients with chronic renal failure (CRF) have altered LBCL and if dialysis itself affects ROS production in the blood. Materials and Methods: Twenty-six HD patients, 11 non-dialyzed patients with CRF, and 20 gender- and age-matched healthy controls were studied. Resting (rCl) and 210?5 M n-formyl-methionyl-leucyl-phenylalanine-stimulated LBCL (peak chemiluminescence: pCl, total light emission after agonist addition: tCl) calculated per 104 phagocytes present in the 3-l blood samples were measured with a Bio-Orbit? 1251 luminometer at 37?C for 11 min. Results: Prior to the HD session, median rCL, pCL, and tCL were 1.5, 3.0, and 2.8 times higher in HD patients than in healthy controls (p<0.01) and tended to increase at the end of the session. Significant increases in tCl were observed at 30 min and 240 min (end) of HD (1023.5 vs. 1810.6 vs. 2006.8 arbitrary unitss/104 phagocytes, n=9, p<0.05). Median pCl and tCl were 5.0 and 4.3 times higher in non-dialyzed patients with CRF than in healthy controls (p<0.001). However, no significant differences were found between pre- and post-HD LBCL of HD patients and the LBCL of non-dialyzed patients with renal failure. Conclusions: Blood from patients with renal failure generates elevated amounts of oxidants independently of HD treatment. This may add to the understanding of the nature of oxidative stress and suggests the need of antioxidant treatment in these patients.
4
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Structures in Multicomponent Polymer Films: Their Formation, Observation and Applications in Electronics and Biotechnology

73%
Budkowski A., Bernasik A., Moons E., Lekka M., Zemła J., Jaczewska J., Haberko J., Raczkowska J., Rysz J., Awsiuk K.
Acta Physica Polonica A
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2009
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vol. 115
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issue 2
435-440
EN
Several strategies to form multicomponent films of functional polymers, with micron, submicron and nanometer structures, intended for plastic electronics and biotechnology are presented. These approaches are based on film deposition from polymer solution onto a rotating substrate (spin-casting), a method implemented already on manufacturing lines. Film structures are determined with compositional (nanometer) depth profiling and (submicron) imaging modes of dynamic secondary ion mass spectrometry, near-field scanning optical microscopy (with submicron resolution) and scanning probe microscopy (revealing nanometer features). Self-organization of spin-cast polymer mixtures is discussed in detail, since it offers a one-step process to deposit and align simultaneously domains, rich in different polymers, forming various device elements: (i) Surface segregation drives self-stratification of nanometer lamellae for solar cells and anisotropic conductors. (ii) Cohesion energy density controls morphological transition from lamellar (optimal for encapsulated transistors) to lateral structures (suggested for light emitting diodes with variable color). (iii) Selective adhesion to substrate microtemplates, patterned chemically, orders lateral structures for plastic circuitries. (iv) Submicron imprints of water droplets (breath figures) decorate selectively micron-sized domains, and can be used in devices with hierarchic structure. In addition, selective protein adsorption to regular polymer micropatterns, formed with soft lithography after spin-casting, suggests applications in protein chip technology. An approach to reduce lateral blend film structures to submicron scale is also presented, based on (annealed) films of multicomponent nanoparticles.
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