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1
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Translational readthrough of a termination codon in the yeast mitochondrial mRNA VAR1 as a result of mutation in the release factor mRF1.

100%
Claisse M. L., Boguta M., Zagórski W.
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2005
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vol. 52
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issue 1
129-137
EN
Yeast mitochondrial DNA codes for eight major polypeptides. Translation of he mitochondrially encoded polypeptides in strains with mutated mitochondrial release factor, mRF1, was found to result in the synthesis of a novel protein, V2. Different mrf1 alleles were associated with different efficiency of V2p synthesis. Translation of V2p was enhanced by paromomycin. Comparative analysis of peptides resulting from protease digestion indicated that V2p is a derivative of Var1p. According to our hypothesis, V2p represents a readthrough product of the natural stop codon in VAR1 mRNA.
2
Content available remote

Digoxigenin-labelled molecular probe for the simultaneous detection of three potato pathogens: potato spindle tuber viroid (PSTVd), potato virus Y (PVY), and potato leafroll virus (PLRV)

100%
Wełnicki M., Żekanowski C., Zagórski W.
Acta Biochimica Polonica
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1994
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vol. 41
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issue 4
473-475
3
Content available remote

Isolation of wheat germ ribosomes free of high molecular weight inhibitors of the natural messenger translation

100%
Rychlik W., Matie-Zabala M., Zagórski W.
Acta Biochimica Polonica
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1979
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vol. 26
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issue 1-2
115-123
4
Content available remote

Kolejny wielki genom poznany przy udziale polskich laboratoriów genom ziemniaka zsekwencjonowany

100%
Gromadka R., Gawor J., Szczęsny P., Zagórski W.
Kosmos
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2011
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vol. 60
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issue 3-4
491-497
PL
W połowie lipca 2011, w Nature ukazał się artykuł "Sekwencja genomu ziemniaka i jej analiza" autorstwa Konsorcjum Sekwencjonowania Genomu Ziemniaka (PGSC). W skład tego Konsorcjum wchodziły 32 zespoły z 14 krajów - znaczących producentów ziemniaków. Współautorami ze strony polskiej, tej wielo autorskiej pracy (94 badaczy, 25 zespołów wiodących) byli członkowie zespołu z Instytutu Biochemii i Biofizyki PAN. Prace Konsorcjum, rozpoczęte w 2007 roku, wspierane były przez rządy państw uczestniczących w programie, w tym Polskiego Ministerstwa Nauki i Szkolnictwa Wyższego, (projekt 47/PGS/2006/01). To trzeci wielki genom (po S. cerevisiae i P. caudatum) w którego poznaniu uczestniczyli pracownicy IBB PAN. Konsorcjum zsekwencjonowało dwa szczepy ziemniaka DM1-3 516 R44 and RH 89-039-16. Szczep DM zsekwencjonowano metodą "shotgun" wykorzystując m. in. platformę sekwencjonowań genomowych IBB PAN (sekwenator Roche GS FLX Titanium 454). Metodami ab initio określono zasób genów kodowanych przez otrzymane sekwencje. Wyniki zweryfikowano analizami transkryptomu objawiającego się w różnych tkankach, czy stadiach rozwojowych oraz w wyniku kontrolowanego stresu. Sumarycznie zidentyfikowano w otrzymanej sekwencji 39031 genów kodujących białka. 25,3% tych genów koduje transkrypty mogące podlegać alternatywnemu składaniu, a więc kontrolujące średnio 2-3 odmienne białka. Wydaje się więc, że w genomie ziemniaka zakodowana jest informacja na temat syntezy ok. 100,000 różnych białek. Porównanie sekwencji DM and RH dowodzi, że ziemniak to roślina wysoce heterozygotyczna. Mutacje punktowe (SNP) występują średnio co 40 nukleotydów, a insercje lub delecje co 394 pary zasad. Wskazuje to wyraźnie, że genom ziemniaka jest niestabilny, co zapewne znajduje swoje odbicie w łatwej degeneracji odmian uprawnych.
EN
In mid-July 2011 Nature published the paper "Genome sequence and analysis of the tuber crop potato" by the Potato Genome Sequencing Consortium - PGSC. This international consortium consisted of 32 teams from 14 countries that are significant potato producers are active in potato breeding programs. The Polish team, representing the PAS Institute of Biochemistry and Biophysics, co-authored the paper, which was signed by 94 consortium members from 25 leading institutions. The work, begun in 2007, was funded by the participating countries' governments - including Poland's Ministry of Science and Higher Education - within the 47/PGS/2006/01. After the yeast and paramecium genomes this is the third large genome sequencing in which IBB teams have participated. Consortium sequenced two strains of potato DM1-3 516 R44 and RH 89-039-16. The DM strain was sequenced by the consortium using the "Shotgun method" on genome sequencing platforms, one of which was created in Warsaw (Roche GS FLX Titanium 454 sequencer).The second strain sequenced was a laboratory S. tuberosum heterodiploid RH 89-039-16. Ab initio predictions of genes and their functions were verified by RNA transcriptome analysis done for various tissues, development stages and under stress. This allowed for the identification of 39031 gene-coding proteins. 25,3% of these genes produce RNA that undergoes splicing, coding for an average of 2-3 different proteins. It therefore seems that the potato genome codes for approximately 100 000 different proteins. Comparisons of the DM and RH sequences show that the potato exhibits high heterozygosity. Single-nucleotiste polymorphisms (SNP) are encountered on average every 40 nucleotides, while insertions or deletions (so-called indel) of an average length of 12.8 nucleotides are encountered on average every 394 base pairs. This data clearly shows that gene damage in the potato genome is a frequent occurrence, which accounts for the easy degeneration of industrial strains.
5
Content available remote

Phosphorylation of wheat germ ribosomes in vitro by wheat germ protein kinase

100%
Chroboczek J., Madjar J.-J., Rychlik W., Zagórski W.
Acta Biochimica Polonica
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1982
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vol. 29
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issue 1-2
135-141
6
Content available remote

Genetic variability of potato spindle tuber viroid RNA replicon.

100%
Góra-Sochacka A., Candresse T., Zagórski W.
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2001
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vol. 48
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issue 2
467-476
EN
The genetic continuity of the potato spindle tuber viroid (PSTVd) genome was analysed after infection of tomato plants with cloned cDNAs of parental strains. During the six weeks of the experiment, several new sequence variants appeared. The sequence variants detected in the progeny population induced sequence-specific disease symptoms. The PSTVd genome therefore follows the pattern expected for typical pseudo-strains propagating in plants as a population of similar sequences. Assessing further the replicon continuity, a PSTVd cDNA mutant with a deletion in the central conserved region was constructed and proven to be non-infectious. Surprisingly, in a sub-population of potato transformants expressing the same deleted PSTVd RNA an infectious viroid was detected. This suggests specific transcript conversion followed by recovery of the full-length pathogen genome.
7
Content available remote

Co-inoculation with two non-infectious cDNA copies of potato spindle tuber viroid (PSTVd) leads to the appearance of novel fully infectious variants.

100%
Podstolski W., Góra-Sochacka A., Zagórski W.
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2005
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vol. 52
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issue 1
87-98
EN
Potato spindle tuber viroid (PSTVd) is one of the smallest (about 360 nt) infectious plant agents. It is composed of a single-stranded circular non-coding RNA molecule. In the course of previous passage experiments with two intermediate PSTVd variants I2 and I4, three non-infectious clones (I2-50, I4-37 and I4 VI-17) were found. When inoculated separately as cDNAs on tomato "Rutgers" test plants these variants did not induce any visible disease symptoms and did not produce progeny. The presence of such non-infectious variants raises several questions about their origin and biology and to answer them, mixed co-infections with cDNA copies of two non-infectious variants (I2-50, I4-37) were performed. PSTVd infection was observed in seven out of 30 inoculated plants. The progeny isolated from three separate plants contained novel variants, together with the parental I2 and I4 sequences. It is conceivable that the appearance of repaired PSTVd molecules, clearly capable of cell-to-cell movement leading to the systemic infection, results from recombination events. An analysis of the recombinant molecules and comparison with databases identified the specific sites responsible for the restricted infectivity of the I2-50 and I4-37 PSTVd variants. In parallel experiments in which (+) strand PSTVd infectious transcripts were used, no recombinants were observed, and the original I2-50 and I4-37 non-infectious sequences were not detected in the progeny.
8
Content available remote

Protein synthesizing system from wheat germ. Efficient translation of synthetic and natural messages

99%
Rychlik W., Zagórski W.
Acta Biochimica Polonica
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1978
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vol. 25
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issue 2
129-146
9
Content available remote

Protein composition of Saccharomyces cerevisiae mitochondrial ribosomes

87%
Mieszczak M., Kozłowski M., Zagórski W.
Acta Biochimica Polonica
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1988
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vol. 35
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issue 2
105-118
10
Content available remote

Nucleotide sequence of RNA of a Polish isolate of potato leafroll luteovirus

76%
Pałucha A., Sadowy E., Kujawa A., Juszczuk M., Zagórski W., Hulanicka D.
Acta Biochimica Polonica
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1994
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vol. 41
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issue 4
405-414
11
Content available remote

Analysis of potyvirus terminal protein VPg-transgenic Arabidopsis thaliana plants

76%
Wojtal I., Piontek P., Grzela R., Jarmołowski A., Zagórski W., Chroboczek J.
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2011
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vol. 58
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issue 3
349-353
EN
Virus-coded VPg protein of Potato virus Y (PVY) does not have homologs apart from other VPgs. Since VPg is indispensable for the potyvirus life cycle, it appeared a good candidate for eliciting pathogen-derived resistance to PVY. Following agroinfection used to obtain PVY VPg-transgenic Arabidopsis thaliana plants, only few transgenic seeds were recovered giving rise to six transgenic plants that contained the VPg gene with the correct sequence. They generated VPg mRNA, but VPg protein was not detected. Some plants were immune to PVY infection suggesting post-transcriptional gene silencing. However, the likely PVY VPg toxicity exerted at an early stage of transformed seeds development precludes its use for engineering pathogen-derived resistance.
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