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SNP-minisequencing as an excellent tool for analysing degraded DNA recovered from archival tissues

100%
Bąbol-Pokora K., Berent J.
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2008
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vol. 55
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issue 4
815-819
EN
SNP-minisequencing has become common in forensic genetics, especially for analysing degraded or low copy number DNA (LCN DNA). The aim of this study was to examine the usefulness of five SNP (single nucleotide polymorphism) markers for analyzing degraded and LCN DNA recovered from archival samples. DNA extractions of eight formalin-fixed paraffin-embedded (FFPE) tissues were performed and DNA fragments were amplified in one multiplex PCR (polymerase chain reaction). SNPs were identified in a minisequencing reaction and a gel electrophoresis in ABI Prism® 377 Sequencer. The research confirmed the usefulness of SNP-minisequencing for analysing FFPE tissues.
2
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Preventable Deaths Among Trauma Patients

61%
Kołodziejczyk-Wojtczak D., Barzdo M., Balcerzyk-Barzdo E., Gaszyński W., Berent J., Piotrowski D.
Polish Journal of Surgery
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2008
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vol. 80
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issue 3
139-145
EN
The aim of the study was to assess the effectiveness of the medical rescue service in the city of Łódź and its surrounding areas.Material and methods. The records of the forensic-medical postmortems conducted at the Institute of Forensic Medicine Medical University in Łódź in 2004 were analyzed. For further analysis, the cases of adults who died of mechanical injury resulting from an accident were chosen. Taking into consideration the autopsy diagnosis, the degree of the individual injuries was defined with the use of the Abbreviated Injury Scale (AIS). Next, the Injury Severity Score (ISS) and the New Injury Severity Score (NISS) were calculated. In cases where specialist medical assistance was given, the medical documentation was analyzed, and the clinical condition of the injured was assessed using the Revised Trauma Scale (RTS), followed by estimation of the probability of survival with the use of the TRISS (Trauma Revised Injury Severity Score).Results and conclusions. Among the analyzed 194 cases of death caused by a mechanical injury resulting from an accident, 19 cases (9.8%) were recognized as preventable (ISS 0-14 or/and NISS 0-14 and/or TRISS >75%). It was established that the deaths assessed as preventable were caused by early or late injury complications, which even correct treatment cannot always prevent.
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