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1

Segregation of transferrin alleles in a flock of long-wool sheep

100%
Kmiec M.
Genetica Polonica
|
1993
|
vol. 34
|
issue 3
273-285
EN
Studies on in the flock of from the State Animal Breeding Station in Bobrowniki were carried out. Genetic structure, of the population consisting of 6832 animals (171 rams, 3050 ewes and 3611 lambs) was examined. Inheritance of was traced and it is postulated to apply studies on transferrin polymorphism for parentage cintrol. In the studied sheep population the most frequent were alleles and BC, BD, CD and BB phenotypes. An analysis of transferrin alleles transfer from parents to their offspring in some cases showed significant divergence between the observed and expected numbers of offspring carrying particular genes. That concerned the phenotype AB in rams as well as AD and BE phenotypes in ewes. A theoretical probability of parentage exclusion in the examined stud of long-wool sheep estimated on the basis of the frequency of genes determining transferrin phenotypes was 43.18%.
2

PCR-RFLPs within the lactate dehydrogenase (LDH-A) gene of the domestic pigeon (Columba livia var. domestica)

63%
Dybus A., Kmiec M.
Journal of Applied Genetics
|
2002
|
vol. 43
|
issue 4
501-504
EN
A total of 45 racing pigeons were genotyped using PCR-RFLP method. PCR product of the LDH-A gene was amplified according to the Long-PCR procedure. The amplification products were digested with restriction enzymes. PCR-RFLPs for two restriction enzymes, HaeIII and NlaIV, were observed. Two pairs of alleles LDH-AA and LDH-AB for LDH-A-NlaIV polymorphism and LDH-AC and LDH-AD for LDH-A-HaeIII polymorphism were detected in the homozygous and heterozygous states. Frequencies of alleles were as follows: A ? 0.622, B ? 0.378 and C ? 0.256, D ? 0.744.
3

Associations between the prolactin receptor gene polymorphism and reproductive traits of boars

63%
Kmiec M., Terman A.
Journal of Applied Genetics
|
2006
|
vol. 47
|
issue 2
139-141
EN
The prolactin receptor gene (PRLR), located on chromosome 16 in pigs, is a candidate gene for reproductive traits. The experiment was aimed to detect the DNA mutations in this gene and to find probable relations between the genotype and some reproductive traits in boars. The polymorphism in the PRLR gene was identified by PCR-RFLP method using specific primers and the restriction enzyme AluI. In total 229 boars of various breeds were genotyped. The frequency of allele A was estimated at 0.62 and allele B at 0.38. Genotype AA was found at a frequency of 0.45, AB at 0.35 and BB at 0.20. We found associations between PRLR genotype and ejaculate volume, sperm concentration, percentage of live sperm, and number of live sperm in the ejaculate (P < 0.01).
4

Single nucleotide polymorphisms in exon 10 of the chinchilla growth hormone receptor (GHR) gene

51%
Polasik D., Kmiec M., Liefers S., Terman A.
Journal of Applied Genetics
|
2005
|
vol. 46
|
issue 4
403-406
EN
The aim of this study was to detect SNPs in exon 10 of the chinchilla growth hormone receptor gene (GHR) by comparative sequencing. Sixty females of the same breed (Standard) were analysed. Four new SNPs were identified, which cause 3 amino acid substitutions in the intracellular domain of the receptor: G/C at position 135 bp (in relation to the total sequence of exon 10) (gln/his), CAG/AAA at 352 bp and 354 bp (gln/lys), and C/A at 641 bp (thr/asn).
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