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Molecular cloning and sequencing of partial cDNA of tumor necrosis factor and p75 tumor necrosis factor receptor of Syrian golden hamster (Mesocricetus auratus) with the use of universal primers.

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Dobrzańska Z., Więckiewicz J., Bigda J.

vol. 49

issue 2

427-431

In this study we cloned and analysed partial cDNA of tumor necrosis factor (TNF) and p75 TNF-R receptor of Syrian golden hamster (Mesocricetus auratus). We obtained a 382-bp sequence of TNF and a 148-bp sequence coding for p75 TNF-R. The primers used for the cloning were designed on the basis of inter-species homology, thus presumably can be used for cloning and analysis of TNF and p75 TNF-R genes of other mammals.

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1 Acta Biochimica Polonica

1 2002

1 Bigda J.

1 Dobrzańska Z.

1 Więckiewicz J.

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Molecular cloning and sequencing of partial cDNA of tumor necrosis factor and p75 tumor necrosis factor receptor of Syrian golden hamster (Mesocricetus auratus) with the use of universal primers.