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Brasinosteroidy w rolnictwie, ogrodnictwie i kulturach in vitro

100%
Janeczko A.
Kosmos
|
2005
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vol. 54
|
issue 2-3
259-265
EN
Summary Brassinosteroids (BR) were found in rape pollen in 1979. These compounds, such as brassinolide, 24epibrassinolide, castasterone are considered to be a new class of phytohormones. BR belong to steroids, a group of compounds, which have a basic sterane carbon skeleton. BR exhibit a multitude of physiological activities. Application of BR cause higher production of plant biomass, an increase in the quality and yield of different economically important crops, such as wheat, potato, rice, etc. Brassinosteroids are natural plant constituents which makes them environmental friendly regulators in agricultural and horticultural production. In in vitro culture, BR stimulate callus formation, plant regeneration and processes of embryogenesis, their application makes protocols of micropropagation of plants more efficient. The physiological activity of BR is still a field of intensive investigations and the most interesting results of which are patented.
2
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Specific binding sites for progesterone and 17β-estradiol in cells of Triticum aestivum L.

51%
Janeczko A., Budziszewska B., Skoczowski A., Dybała M.
|
2008
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vol. 55
|
issue 4
707-711
EN
The presence and location of specific binding sites for progesterone and 17β-estradiol in cells of wheat were estimated using radioligand binding assay. Membrane and cytosolic fractions of non-vernalized and vernalized plants were tested using tritium-labelled ligands. Specific binding of [3H]progesterone and [3H]17β-estradiol occurs in wheat cells. The binding sites are located in membranes and in the cytosol. Specific binding of [3H]17β-estradiol is higher in the membranes than in the cytosol. Specific binding of both ligands in the cytosolic fraction is higher in vernalized plants than in non-vernalized ones. The possibility of the occurrence of steroid binding proteins specific for progesterone and 17β-estradiol, putative steroid receptors for these steroids in Triticum aestivum L., is discussed.
3
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FT-Raman spectroscopy as a tool in evaluation the response of plants to drought stress

45%
Rys M., Szaleniec M., Skoczowski A., Stawoska I., Janeczko A.
Open Chemistry
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2015
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vol. 13
|
issue 1
EN
The aim of study was to evaluate the usefulness of FT-Raman spectroscopy in assessing stress-induced metabolic changes in plants. 20-d-old optimally watered plants of soybean were exposed to drought. Metabolic changes in optimally watered and drought-stressed plants were monitored using FT-Raman spectroscopy. In parallel, analyses were carried out of fatty acid composition and pigment content using analytical methods. These compounds are associated with the response of plants to environmental stress. While fatty acid assays in study were inconclusive, the pigment content analysis gave promising results. FT-Raman experiment demonstrated a decrease in carotenoid content in leaf, as a result of drought, which was confirmed by spectrophotometric analysis. In addition to the analysis of aforementioned compounds, FT-Raman spectroscopy allowed the simultaneous observation of a wider spectrum of compounds scattering the radiation in the leaves tested, and their subsequent comparative analysis. The impact of drought on metabolism of soybean was clearly visible on spectra and confirmed using cluster analysis. The technical problem of the influence of leaf water content on measurements, which appeared in studies, will be discussed. To conclude, FT-Raman spectroscopy may be a good complement to other non-invasive methods, e.g., fluorescent methods, in assessing the stress-induced damage of crops.
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