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The influence of various light spectra on the efficiency of somatic embryogenesis

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Menke-Milczarek I., Zebrowski J., Zimny J.
Biotechnologia
|
2001
|
issue 2
99-103
EN
The aim of presented work was to investigate the impact of various light spectra on the efficiency and intensity of wheat somatic embryogenesis. The efficiency of somatic embryogenesis was defined as a percentage of explants forming somatic embryos in reference to all cultured explants. Immature embryos at the spherical coleoptile stage were excised from seeds of a few varieties of wheat and placed onto MS medium (1962) supplemented with 30 muM Dicamba. The influence of blue, white and red light on the callus growth and induction of somatic embryogenesis was compared. Increase in proportion between the red (600-700nm) and blue (400-500nm) component of light spectrum accelerated development of somatic embryos and increased the efficiency of somatic embryogenesis.
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