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1

Description of the mallard duck (Anas platyrhynchos) karyotype

100%
Wojcik E., Smalec E.
Folia Biologica
|
2007
|
vol. 55
|
issue 3-4
115-120
EN
The karyotype of the mallard duck, Anas platyrhynchos, was characterised on the basis of R and C bands. Chromosomal preparations obtained from in vitro blood lymphocyte cultures were RBG- and CBG-stained. The structures of nine and 14 pairs of chromosomes were analysed by the RBG and CBG chromosome banding techniques, respectively. The location of R bands, as well as the size and arrangement of constitutive heterochromatin blocks were determined. Ideograms of R and C banded patterns of the analysed chromosomes were drawn. The morphological makeup of the analysed chromosomes was assessed.
2

Description of the Anser cygnoides goose karyotype

100%
Wojcik E., Smalec E.
Folia Biologica
|
2008
|
vol. 56
|
issue 1-2
37-42
EN
The karyotype of the domestic goose A. cygnoides was characterised on the basis of R and C bands. Chromosomal preparations obtained from an in vitro culture of blood lymphocytes were stained by means of the RBG and CBG banding techniques. The first nine pairs of chromosomes were analysed by the R banding technique, while fourteen pairs of chromosomes were analysed by the C banding technique. The localisation of R bands as well as the sizes and positions of constitutive heterochromatin blocks were determined. Ideograms of R and C banded patterns for the analysed chromosomes were drawn. The morphological make-up of the analysed chromosomes was assessed.
3

Description of the Anser anser goose karyotype

100%
Wojcik E., Smalec E.
Folia Biologica
|
2007
|
vol. 55
|
issue 1-2
35-40
EN
The karyotype of the Italian goose originating from Anser anser was characterised on the basis of R and C bands. Chromosomal preparations obtained from an in vitro culture of blood lymphocytes were stained with the RBG and CBG techniques. The RBG technique enabled the analysis of the structure of nine pairs of chromosomes whereas the CBG technique ? fourteen pairs of chromosomes from the total of eighty goose chromosomes. The morphology and the R and C banding patterns were described. The size and arrangement of the blocks of constitutive chromatin were determined. Ideograms of R and C banded patterns were drawn. The morphological structure of the analysed chromosomes was evaluated.
4

Description of the Muscovy duck (Cairina moschata) karyotype

100%
Wojcik E., Smalec E.
Folia Biologica
|
2008
|
vol. 56
|
issue 3-4
243-248
EN
The karyotype of theMuscovy duck originating from Cairina moschata was characterised on the basis of R and C bands. Chromosomal preparations obtained from an in vitro blood lymphocyte culture were RBG- and CBG-stained. The structures of nine and fourteen pairs of chromosomes were examined based on R bands and C bands, respectively. Ideograms of banded patterns of the analysed chromosomes were drawn. The sizes of individual constitutive heterochromatin blocks wereas measured. The morphology of the analysed chromosomes was assessed.
5

Nucleotide sequence polymorphism within exon 4 of the bovine prolactin gene and its associations with milk performance traits

81%
Brym P., Kaminski S., Wojcik E.
Journal of Applied Genetics
|
2005
|
vol. 46
|
issue 2
179-185
EN
Prolactin plays an important regulatory function in mammary gland development, milk secretion, and expression of milk protein genes. Hence the PRL gene is a potential quantitative trait locus and genetic marker of production traits in dairy cattle. We analysed the sequence of the PRL gene to investigate whether mutations in this sequence might be responsible for quantitative variations in milk yield and composition. Using SSCP and direct sequencing, we detected six single-nucleotide polymorphisms within a 294-bp prolactin gene fragment involving exon 4. All detected mutations were silent with respect to the amino acid sequence of the protein. PCR-RFLP genotyping of SNP 8398 R (RsaI) was used to assess allele frequencies in 186 Black-and-White cows (0.113 and 0.887 for A and G, respectively) and in 138 Jersey cows (0.706 and 0.294 for A and G, respectively). Black-and-White cows with genotype AG showed the highest milk yield, while cows with genotype GG showed the highest fat content.
6

MilkProtChip ? a microarray of SNPs in candidate genes associated with milk protein biosynthesis ? development and validation

71%
Kaminski S., Ahman A., Rusc A., Wojcik E., Malewski T.
Journal of Applied Genetics
|
2005
|
vol. 46
|
issue 1
45-58
EN
MilkProtChip is an oligonucleotide microarray based on the arrayed primer extension (APEX) technique, allowing genotyping of single nucleotide polymorphisms (SNPs) in genes of interest for bovine milk protein biosynthesis. APEX consists of a sequencing reaction primed by an oligonucleotide anchored with its 5'end to a glass slide and terminating one nucleotide before the polymorphic site. The extension with one fluorescently labeled dideoxy nucleotide complementary to the template reveals the polymorphism. A total of 75 SNPs were selected among those associated directly or potentially with milk protein content. Among the 75 SNPs, 4 did not produce a positive signal. Most of the remaining SNPs produced a signal for both strands, except for 4 (one strand). In the validation step, 12 Polish Holstein bulls, 1 Polish Red bull, 1 bison (Bison bonasus), 11 Jersey cows and 25 Polish Holstein cows were screened to validate SNPs. Among the 71 selected SNPs ? 26 were found monoallelic, the rest showing at least two genotypes for the entire population under study. All the animals were earlier genotyped for 2?5 SNPs by PCR-RFLP and PCR sequencing and all showed complete concordance with APEX genotyping. APEX reactions showed relatively high signal frequencies: more than 0.9, 0.9?0.8 and below 0.8, for 65, 4 and 2 DNA samples, respectively. The primary application of the MilkProtChip is the simultaneous genotyping of dozens of SNPs to reveal and clarify the genetic background of milk protein biosynthesis. The chip may possibly be used for dairy cattle identification and paternity analysis, evolutionary studies, the evaluation of genetic distances between wild and domestic cattle breeds and the domestication history of bovine species.
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