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1

Differences in the inheritance stability of kanamycin resistance between transgenic cucumbers (Cucumis sativus L.) containing two constructs

100%
Yin Z., Szwacka M., Malinowski R., Malepszy S.
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issue 3
307-314
EN
A phenotypic segregation of kanamycin resistance conferred by nosnptII was investigated in two groups of transgenic cucumber lines one containing the PR-2duidA-nosnptII construct and the other with the thaumatinII-nosnptII construct to the third and fifth generation, respectively. Expression of the nptII gene was more stable and predictable in the progeny of the PR-2d lines than that of thaumatin lines. In T2 and T3 generations representing seven PR-2d primary transformants, 78% of the hemizygous progenies exhibited a segregation ratio consistent with Mendelian inheritance; non-Mendelian ratio occurred in the remaining 22%. Mendelian segregation was observed in 46% of the hemizygous progenies derived from 11 thaumatin primary transformants. The segregation ratio for two and three independent loci appeared in single PR-2d and thaumatin lines, respectively.
2
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Effects of electron radiation on properties of PLA

100%
Malinowski R., Rytlewski P., Żenkiewicz M.
Archives of Materials Science and Engineering
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2011
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vol. 49
3
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Effects of electron radiation on properties of PLA

100%
Malinowski R., Rytlewski P., Żenkiewicz M.
Archives of Materials Science and Engineering
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2011
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issue 1
4

Transformation of selected genotypes of tomato with the help of A. tumefaciens

88%
Bartoszewski G., Fedorowicz O., Malinowski R., Niedziela A., Niemirowicz-Szczytt K.
Biotechnologia
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2000
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issue 4
99-102
EN
Tomato belongs to important crops widely cultivated all over the world. It is also one of the five most popular vegetables grown in Poland. At the same time, tomato is known to be a model species in modern biology and biotechnology. Since 1985 a lot of reports on tomato transformation with the use of Agrobacterium have been published. Recently, first transgenic varieties of this species have also been developed. Flavr Savr? obtained by Calgene, USA was the first cultivar obtained as a result of genetic engineering, officially registered in the United States. In our Department the methods of tomato transformation with Agrobacterium tumefaciens have been adapted and optimised. Numerous transgenic plants have been obtained, such as commercial varieties (Beta, Potentat), inbred lines as well as tomato mutants (non-ripening, lateral suppressor). The following genes were introduced to the above forms: beta-glucuronidase reporter gene (gusA), thaumatin gene (sweet protein), isopentenyl transferase gene (ipt, coding the key enzyme in cytokinin metabolic pathway) and thus homozygous lines were developed (T2 generation). Most recently, attempts have been made to incorporate mgfp5-ER gene coding green fluorescence protein, nucleoprotein (N) gene from tomato spotted wilt virus (TSWV) and cDNA of putative P450 cytochrome (CYP72) in order to test gene expression and interaction.
5

Tissue-culture-responsive and autotetraploidy-responsive changes in metabolic profiles of cucumber (Cucumis sativus L.)

76%
Filipecki M., Yin Z., Wisniewska A., Smiech M., Malinowski R., Malepszy S.
Journal of Applied Genetics
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2006
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vol. 47
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issue 1
17-21
EN
Somaclonal variation commonly occurs during in vitro plant regeneration and may introduce unintended changes in numerous plant characters. In order to assess the range of tissue-culture-responsive changes on the biochemical level, the metabolic profiles of diploid and tetraploid cucumber R1 plants regenerated from leaf-derived callus were determined. Gas chromatography and mass spectrometry were used for monitoring of 48 metabolites and many significant changes were found in metabolic profiles of these plants as compared to a seed-derived control. Most of the changes were common to diploids and tetraploids and were effects of tissue culture. However, tetraploids showed quantitative changes in 14 metabolites, as compared to regenerated diploids. These changes include increases in serine, glucose-6P, fructose-6P, oleic acid and shikimic acid levels. Basing on this study we conclude that the variation in metabolic profiles does not correlate directly with the range of genome changes in tetraploids.
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