Full-text resources of PSJD and other databases are now available in the new Library of Science.
Visit https://bibliotekanauki.pl
Preferences help
Polish version English version Language
enabled [disable] Abstract
Number of results

Results found: 2

Number of results on page
first rewind previous Page / 1 next fast forward last

Search results

help Sort By:

help Limit search:
first rewind previous Page / 1 next fast forward last
1

Micropropagation of sweet cherry rootstocks

100%
Dziedzic E., Malodobry M.
Biotechnologia
|
2004
|
issue 2
206-211
EN
The sweet cherry rootstocks Gisela 5, Weiroot 10, Damil, Edabriz, Maxma, PHL 84 were propagated and rooted by tissue culture. The micropropagation was carried out on MS medium (Murashige and Skoog, 7) with modification. Medium A ? full strength MS with addition of 0,5 mg/l BA and 0,1 mg/l IBA, medium B ?half strength of MS nitro-elements with addition of 2,0 mg/l BA and 0,1 mg/l IBA. Two-steps rooting was carried out on WPM medium (Lloyd and McCown, 8), induction of roots on WPM medium with addition of 2,0 mg/l IBA and 5,0 mg/l IAA, after 9-10 days shoots were transferred onto WPM medium without hormones. The highest multiplication coefficient was obtained for Weiroot 10. Gisela 5 was proved to be the most susceptible to vitrification. The best rooting was calculated for Gisela 5-94,7%, at mean length of root ? 3,2 cm.
2

Early stages of embryogenesis after fertilization in vitro condition

81%
Dziedzic E., Lech W., Malodobry M.
Biotechnologia
|
2001
|
issue 2
54-56
EN
Ovules of plum cultivars (Sweet Common Prune, Herman, Gilbert, Sanctus Hubertus, Cacanska Rodna, Cacanska Najbolia) were pollinated with pollen of cv. Stanley on the White?a medium with addition of 15% sucrose. The nucelluses excised from the fertilized ovules were cultivated on Norstog medium with addition of 3,4% sucrose. The process of fertilization after pollination in the in vitro condition was proved on the basis of the paraffin cross?sections: the degeneration of synergid after entering the pollen tube, the fertilization of egg cell were confirmed. The microscopic observations showed the swelling of micropylar part of nucellus. The paraffin cross-sections showed a many-layer kalotka (peculiar morphological structure) and swelled cells of nucellus among which the embryo sac was observed. The presence of embryo without endosperm or the presence of endosperm without embryo in the embryo sac was noted.
first rewind previous Page / 1 next fast forward last
JavaScript is turned off in your web browser. Turn it on to take full advantage of this site, then refresh the page.