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Apoptosis in relation to neuronal loss in experimental Creutzfeldt-Jakob disease in mice

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Jesionek-Kupnicka D., Kordek R., Buczynski J., Liberski P. P.
Acta Neurobiologiae Experimentalis
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2001
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vol. 61
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issue 1
13-19
EN
Apoptosis constitutes a genetically determined process to eliminate superfluous or damaged cells in tissues. Deficiencies in apoptosis regulation are involved in different pathologies including prion diseases. Some experimental studies show that neuronal loss - one of the hallmarks of prion diseases may be accomplished by apoptosis. We evaluated twenty five mice infected experimentally with the Fujisaki strains of CJD and sacrified sequentially in one week intervals. Apoptotic cells in various brain regions were detected by in situ end labelling (TUNEL) and electron microscopy in comparison with neuronal cell loss. The number of labelled cells per brain was very low - from a few labelled cells 6 weeks after inoculation to a maximum of 14 in the terminal stage. The number of neurones counted in 8 selected areas were considerably lower in terminally sick animals (20 and 21 week of incubation period) than in control mice. The mean value of loss of neuronal cells was 32%. The greatest loss (55%) of neurones was noted in the septal nuclei of the paraterminal body and the least lost (16%) in the hypothalamus. Compared to the extensive neuronal loss (30-50%), the number of apoptotic cells detected by in situ end labelling seems to be very low, and the process of neuronal death become more intensive during the progression of the disease.
2

Langerhans cells in vulvar lichen sclerosus and vulvar squamous cell carcinoma

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Rotsztejn H., Trznadel-Budzko E., Jesionek-Kupnicka D.
Archivum Immunologiae et Therapiae Experimentalis
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2006
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vol. 54
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issue 5
363-366
EN
Introduction: Langerhans cells (LCs), specializing in antigen presentation, are a very important part of the skin immune system (SIS). Materials and Methods: Skin biopsies from 22 women with vulvar lichen sclerosus (LS): 15 patients with early and 7 with the late stage of the disease, were evaluated. Five women with vulvar squamous cell carcinoma (SCC) were also examined. The control group consisted of 9 women who underwent plastic surgery of the vulvar region. Immunohistochemical staining was performed on formalin-fixed paraffin-embedded tissues samples using antihuman CD1a antibody (NCL-CD1a-235, Novocastra). Results: Increased numbers of LC stainings were present in early LS, whereas decreased numbers of these cells were present in late LS and in SCC compared with the control group. Conclusions: This study showed that dysregulation of the SIS may lead to suppression of LCs in the vulvar epithelium and may be one of the reasons for a higher tendency for carcinogenesis in the vulvar region.
3

Do Langerhans cells play a role in vulvar epithelium resistance to squamous cell carcinoma?

100%
Rotsztejn H., Trznadel-Budzko E., Jesionek-Kupnicka D.
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2007
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vol. 55
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issue 2
127-130
EN
Introduction: Langerhans cells (LCs) are a very important part of the skin immune system. Materials and Methods: Skin biopsies taken from 13 women after the removal of vulvar squamous cell carcinoma (SCC) who had not been treated earlier for any vulvar diseases were investigated. The control group consisted of 12 women who underwent a plastic surgical operation of the vulva region. Immunohistochemical staining was performed on formalin-fixed, paraffin-embedded tissues samples using antihuman CD1a antibody (NCL-CD1a-235, Novocastra). Results: This study showed a large decrease in LCs in vulvar SCC. Conclusions: It is postulated that the reduction in the number of LCs may be one of the reasons for a higher tendency of carcinogenesis in the vulvar region. Their role as a main element of the skin immune system in the initiation of this process needs further investigation. It is possible that research on LCs in the skin will cast a new light on their role and even contribute to the prophylaxis and treatment of skin and mucosa carcinomas.
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