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Determination of Total Phenolic Content and Some Selected Metals in Extracts of Moringa oleifera, Cassia tora, Ocimum gratissimum, Vernonia baldwinii and Telfairia occidentalis Plant Leaves

100%
Louis H., Maitera O. N., Boro G., Barminas J. T.
World News of Natural Sciences
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2017
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vol. 11
11-18
EN
The main objective of this research is to determine the content of metals (Ca, Cu, Fe, Mg and Zn) and total phenols in different plant extracts of Moringa oleifera, Cassia tora, Ocimum gratissimum, Vernonia baldwinii and Telfairia occidentalis. Content were determined using Atomic Absorption Spectroscopy. The result indicate that Moringa oleifera plant extracts range from 0.25 ±0.00 to 6.13 ±0.30 mg/kg, Cassia tora plant extracts - 0.17 ±0.03 to 7.48 ±0.06 mg/kg, Ocimum gratissimum plant extracts - 0.18 ±0.00 to 5.43 ±0.12 mg/kg, Vernonia baldwinii and Telfairia occidentalis plant extracts - 0.21 ±0.03 to 7.86 ±0.12 mg/kg and 0.17 ±0.00 to 4.52 ±0.06 mg/kg, respectively. The results also revealed a lower abundance of heavy metals. The total phenolic content was determined using the modified Folin-Ciocalteu method. Herein, the phenolic content in Moringa oleifera was 8.50 ±1.23 mg Garlic Acid Equivalent g-1 (mg GAE g-1), Cassia tora - 30.00 ±0.00 mg GAE g-1, Ocimum gratissimum - 45.00 ±1.41 mg GAE g-1 , Vernonia baldwinii - 49.00 ±1.14 mg GAE g-1 and Telfairia occidentalis - 46.6 7 ±0.27 mg GAE g-1. We found the lowest total phenol content in Moringa oleifera. This also possessed high chelating activity. In contrast, Vernonia baldwinii contained the highest total phenol content, but had low chelating activity. The plant extracts with high levels of phenolic compounds exhibited good antioxidant activity.
2
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Photochemical screening and corrosion inhibition of Poupartia birrea back extracts as a potential green inhibitor for mild steel in 0.5 M H2SO4 medium

88%
Louis H., Japari J., Sadia A., Philip M., Bamanga A.
World News of Natural Sciences
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2017
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vol. 10
95-100
EN
Corrosion of metals is a serious environmental problem and has been given much attention in the oil and gas industries. In several industrial processes (acid cleaning and etching, removal of rust and scale), metal surfaces are often made to come in contact with acids. In the present study, an attempt was made to examine Poupartia birrea (P. birres) back extract as a potential green corrosion inhibitor for mild steel in a 0.5 M H2SO4 medium, using the weight loss determination method. The results of our work indicate that highest inhibition efficiency exhibited by Poupartia birrea is 70%. The inhibiting effect of the studied extract could be attributed to the presence of phytochemical constituents present in the extract that are adsorbed on the surface of the mild steel. The plant extract can, hence, be considered as being eco-friendly and an effective green corrosion inhibitor for mild steel exposed to an acid medium.
3
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Prevalence and Antibiotic Susceptibility Patterns of Shigella and Salmonella Causing Diarrhoea in Children Below 5 Years at Thika Level Five District Hospital

88%
Kibet S. C., Matiru V. N., Kibaba P. W., Mucheke A., Louis H.
World News of Natural Sciences
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2017
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vol. 11
28-36
EN
The aim of the study was to isolate and identify the extent of Salmonella and Shigella induced diarrhoea in children under five and to determine their antibacterial susceptibility patterns. The method of investigation was a cross-sectional study. Samples were collected from children less than five years old afflicted with diarrhoea coming for treatment at Thika Level Five Hospital, Kiambu County. A total of 80 stool samples were collected. These were specifically examined for Shigella and Salmonella. In doing so, the samples were cultured in MacConkey and Xylose lactose deoxycholate (XLD) agar. The plates were subsequently incubated aerobically at 37 °C overnight. After incubation, suspected Salmonella and Shigella colonies were identified morphologically, marked and the colonies inoculated to biochemical tests for species identification as described in the Medical Laboratory Manual. Following this, colonies derived from purity plating through biochemical testing were sub-cultured onto nutrient agar to obtain pure colonies. The obtained pure colonies were then used to perform susceptibility tests to commonly prescribed antibacterial drugs, including Gentamicin, Ciproflaxicin and Erythromycin. Results showed 10 (12.5%) confirmed positive isolates, where 6 (60%) were Salmonella and 4 (40%) Shigella. With respect to age, both isolates were found to be concentrated more at ages of 1-4 years. Herein, Salmonella – 1-2 years (33.33%), - 2-3years (50%); Shigella – 1-2 years (50%), - 2-3 years (25%). From the study, sex distribution (male or female) of the host isolates was equal (50-50%). Moreover, Ciprofloxacin and Gentamycin were the most potent antibiotics, whereas Ampicillin, Erythromycin, Cotrimoxazole and Sulfamethoxazole were highly resisted. According to study results, Quinolones and Aminogylcosides are the antibiotics of choice for severe diarrhoea illnesses caused by Salmonella and Shigella.
4
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Production and characterization of biodiesel from coconut extract (Cocos nucifera)

88%
Maitera O. N., Louis H., Dass P. M., Akakuru U. O., Joshua Y.
World News of Natural Sciences
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2017
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vol. 9
62-70
EN
In this study, oil was extracted from coconut. The extracted oil was used to produce biodiesel and the oil and the biodiesel produced were characterised. Biodiesel fuels are alternative diesel fuels usually obtained from renewable sources, mainly, vegetable and animal oils. Oil was extracted from coconuts bought from a local market in Wukari Taraba State, Nigeria, by means of the solvent method. The extracted coconut oil was then characterized. This showed that the coconut oil has a density of 0.91 g, viscosity of 23 mm2/s, saponification value of 191 mg KOH/g, iodine value of 10 mgl2/g, acid value 1of 4.0 mg KOH/g and flash point of 266 °C. The biodiesel of the oil was produced using a transesterification process. The biodiesel was also characterized. Results obtained showed that the biodiesel has a density of 0.89 g, viscosity of 2.83 mm2/s, acid value of 0.18 mg KOH/g, saponification value of 91 mg KOH/g, iodine value of 8 mgl2/g, acid value of 0.18 mg KOH/g and flash point of 110 °C. The physicochemical properties of the biodiesel produce from coconut oil is comparable with that of standard biodiesel in the range of ASTM specifications.
5
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Physico-chemical properties and fatty acid composition of Lagneraria siceraria seed oil

76%
Magu T. O., Sunday E. A., Louis H., Upla P. U., Ukpanah M. A., Nyong B. E.
World News of Natural Sciences
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2017
|
vol. 10
70-79
EN
Oil was extracted from the dehulled seeds of Lagneraria siceraria (bottle gourd) and analysed for physico-chemical properties, as well a fatty acid composition. Standard procedures were employed in all analysis. The seed oil was liquid at room temperature with percentage yield (23.65%). The oil was characterized in terms of specific gravity (0.918 g/cm3), refractive index (1.34), viscosity (26.46 X 103 poise), melting point (11-14.5 °C), moisture content (0.18%), saponification value (203.36 mg KOH/g), unsaponifiable matter (7.13%), iodine value (46.1 g/100g), peroxide value (7.5 meq/kg), free fatty acid value (18.42%), acid value (60.02 mg KOH/g) and ester value (143.34 mg KOH/g). It was also classified as non- drying (iodine value ˂115 g/100 g). The peroxide value indicates that the oil is less prone to rancidity with iodine value less than 30meq/kg. The high saponification value qualifies it for use in the manufacture of soaps and shampoos. Four classes of fatty acid were identified in the oil: palmitic acid (C16:1) (13.5 ± 0.21), stearic acid (C18:1) (6.5 ± 0.96), oleic acid (C18:1) (11.6 ± 0.62) and linoleic acid (C18:2) (68.4 ± 0.13). Linoleic acid was the most abundant fatty acid in the oil. The fatty acid content of the oil reveals that L. Siceraria seed oil could be a rich source of oil for domestic and industrial purposes if exploited.
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