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ANTI-INFLAMMATORY ACTIVITY AND CYTOTOXICITY OF DIOSGENIN ON LIPOPOLYSACCHARIDES INDUCED RAW 264.7 CELLS

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ning j., chang y., wang r., lan z., ru q., liu m., tian c.

Acta Poloniae Pharmaceutica - Drug Research

2020

vol. 77

issue 2

313-317

Diosgenin is a steroidal sapogenin compound, and possesses multiple biological activities including anti-inflammatory, anticancer, immunological regulation, and anti-aging. The current study focused on its anti-inflammatory activities and cytotoxicity by analysis of NO production, phagocytosis activity, secretion of TNF-α and IL-6 and cell viability in LPS-induced RAW 264.7 cells. An IC50 value of diosgenin of 2.8 μM was calculated for diosgenin by regression of cell viability from concentrations ranging from 0.01 to 25 μM; this indicated that 0.01, 0.02, 0.04 μM diosgenin could reduce phagocytic activity very significantly (p<0.01) in a dose-dependent manner with no cytotoxic effect on the LPS-induced RAW 264.7 cells. However, there was no significant effect on NO content and secretion of TNF-α and IL-6 after diosgenin treatment. The research revealed that low concentrations diosgenin can directly inhibit cells phagocytosis, with no effect on the release of inflammatory mediators and cytokines. This lays a foundation for screening for a safe dose in research and developent of derivatives and new formulation of diosgenin for its anti-inflammatory effect.

Dose-effect relationship on anti-inflammatory activity on LPS induced RAW 264.7 cells and antioxidant activity of rutin in vitro

100%

tian c., guo y., chang y., zhao j., cui c., liu m.

Acta Poloniae Pharmaceutica - Drug Research

2019

vol. 76

issue 3

511-522

Rutin, as a common flavonol glycoside, exists abundantly in many plants and exhibits various nutritional and pharmacological function. Current study focused on the dose-effect relationship of rutin on the anti-inflammatory and antioxidant activities in vitro against LPS-stimulated RAW 264.7 cells and free radicals or ferric-ion. This study evaluated the anti-inflammatory activity by observation cell morphological and determination of the content of NO, TNF-α, IL-1β and IL-6, and the phagocytic activity in LPS induced RAW 264.7 cells; and revealed the antioxidant activities via assay of DPPH radical scavenging capacity, ABTS radical scavenging capacity, and reducing power. The study indicated that 5, 10, 20, 50 and 100 μM of rutin all exhibited a better anti-inflammatory activity, in especial 50 and 100 μM. Meanwhile, it exerted a stronger antioxidant activities than BHT, and equivalent to VC at the same concentration. Combined, the results suggested that rutin will be a potential and important adjuvant in treatment of inflammatory diseases and oxidative stress.

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2 Acta Poloniae Pharmaceutica - Drug Research

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2 tian c.

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1 ning j.

1 ru q.

1 wang r.

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ANTI-INFLAMMATORY ACTIVITY AND CYTOTOXICITY OF DIOSGENIN ON LIPOPOLYSACCHARIDES INDUCED RAW 264.7 CELLS

Dose-effect relationship on anti-inflammatory activity on LPS induced RAW 264.7 cells and antioxidant activity of rutin in vitro