Full-text resources of PSJD and other databases are now available in the new Library of Science.
Visit https://bibliotekanauki.pl
Preferences help
Polish version English version Language
enabled [disable] Abstract
Number of results

Results found: 4

Number of results on page
first rewind previous Page / 1 next fast forward last

Search results

help Sort By:

help Limit search:
first rewind previous Page / 1 next fast forward last
1
Content available remote

Nanocomposite Polymer Scaffolds for Bone Tissue Regeneration

100%
Stodolak-Zych E., Frączek-Szczypta A., Wiecheć A., Błażewicz M.
Acta Physica Polonica A
|
2012
|
vol. 121
|
issue 2
518-521
EN
Nanocomposite polymer scaffolds for tissue engineering were prepared using leaching method. As a porogen there were used phosphate salts with different grain size (100-400 μm). Nanocomposite materials based on polylactide (PL/DLA) containing 2 wt% of ceramic bioactive nanoadditives (SiO_2) were prepared. The nanoadditive was characterized by dynamic light scatering (DLS) (size) and the Brunauer-Emmett-Teller (specific surface area) methods. Morphology of the nanoparticles was observed using the transmission electron microscopy. The optimal concentration of the nanofiller in the polymer matrix was evaluated on the basis of in vitro tests of the nanocomposite foils contacted with osteoblast-like human cells of MG63 line. The morphology and porosity of the scaffold after leaching was evaluated using scanning electron microscopy and hydrostatic weighing. The bioactivity test made on the scaffolds demonstrated ability to nucleation of apatite structure on the material.
2
Content available remote

The Study of Human Osteoblast-Like MG 63 Cells Proliferation on Resorbable Polymer-Based Nanocomposites Modified with Ceramic and Carbon Nanoparticles

88%
Wiecheć A., Stodolak-Zych E., Frączek-Szczypta A., Błażewicz M., Kwiatek W.
Acta Physica Polonica A
|
2012
|
vol. 121
|
issue 2
546-550
EN
Polymer-based nanocomposites containing biocompatible and bioactive nanocomponents seem to be excellent materials that could be used in many biomedical applications. The aim of this study was biological evaluation of resorbable polymer-based nanocomposites (PLA, PCL) and their modifications with ceramic nanoparticles (silica - SiO_2, montmorillonite - MMT) or carbon nanotubes. The nanocomposites were seeded with the human osteoblast-like MG 63 cells. After 1, 3 and 7 days of incubation, Trypan blue exclusion test was used to determine the viability and number of cells. The cell population density depending on incubation time and cell population doubling time was calculated. The cell proliferation abilities on the all applied nanocomposites and on control material (polystyrene cell culture plate) were also compared. The number of cells growing on the nanocomposite surfaces increased with the incubation time. The cell viability was not decreased for all applied materials during the entire study (97-100%). The ceramic nanoparticles and carbon nanotubes modified the bone cell growth and proliferation rate. Results of this study confirm that all types of the nanocomposites are appropriate to the growing and proliferation of human osteoblast-like cells.
3
Content available remote

Applications of Comet Assay for the Evaluation of Genotoxicity and DNA Repair Efficiency in Nanomaterials Research

76%
Panek A., Błażewicz M., Frączek-Szczypta A., Adamczyk J., Wiltowska-Zuber J., Paluszkiewicz C.
Acta Physica Polonica A
|
2018
|
vol. 133
|
issue 2
280-282
EN
The single cell gel electrophoresis method, known as comet assay, is a rapid and sensitive technique for testing novel chemicals and nanoparticles for genotoxicity, monitoring environmental contamination with genotoxins and human biomonitoring. In our studies we check the applicability of this method for the evaluation of biocompatibility of modified (MWNF) and non-modified multi-walled carbon nanotubes (MWNT) as well as potential genotoxicity of mercury(II) nitrate. The obtained results enabled us to conclude that the presence of Hg(NO₃)₂ (p<0.001) and MWNT (p<0.04) cause a significantly higher level of DNA damage in comparison to functionalised nanomaterials MWNF. It was implied that for the three investigated agents only mercury significantly enhanced genotoxic effect of X-ray exposure (p<0.001) and inhibition of radio-induced DNA damage repair. On the contrary, the presence of MWNF have no influence on cellular repair efficiencies, while incubation with MWNT causes apoptosis and consequently results in lack of attached cells. In conclusion, our results confirmed the genotoxicity of mercury and non-modified carbon nanotubes as well as the biocompatibility of modified nanotubes. Additionally, we proved the usefulness of comet method for the evaluation of genotoxicity and DNA repair under the influence of different compounds and nanomaterials.
4
Content available remote

Genotoxicity Study of Carbon Nanoforms using a Comet Assay

76%
Panek A., Frączek-Szczypta A., Długoń E., Nocuń M., Paluszkiewicz C., Błażewicz M.
Acta Physica Polonica A
|
2018
|
vol. 133
|
issue 2
306-308
EN
Carbon nanoforms due to their unique properties can be applied in many areas also in medicine. This article presents preliminary genotoxicity studies of electrospun carbon nanofibers (ECNF). This material, apart from its numerous applications, may also be a candidate for use in medical therapy and diagnostics. Polyacrylonitrile (PAN) nanofibers received in the electrospinning process were carbonized and thereafter subjected to oxidation treatment (ECNF-F). Both types of fibres were analyzed with regard to genotoxic influence on the fibroblast line cells using comet assay. Additionally, comet assay experiments were conducted on biocompatible carbon nanotubes with a hydrophilic surface. The results indicate the key role of the oxidation process in the functionalization of carbon nanoparticles intended for medical purposes.
first rewind previous Page / 1 next fast forward last
JavaScript is turned off in your web browser. Turn it on to take full advantage of this site, then refresh the page.