Search results

1

Inhibition of RNA polymerase B activity in normal and regenerating rat liver after administration of 9-aminoacridine

100%

Krawczyk Z.

Acta Biochimica Polonica

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1982

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vol. 29

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issue 3-4

259-273

2

The structural and functional analysis of the human HSPA2 gene promoter region

82%

Pigłowski W., Nowak R., Krawczyk Z., Ścieglińska D.

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2007

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vol. 54

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issue 1

99-106

EN

HSPA2 is a human counterpart of the testis-specific rodent Hst70/Hsp70.2 gene. In contrast to the latter, the expression of the human HSPA2 gene is not limited to the testis, and recent data show that human tumor cells can express this gene at significant levels. The characteristics of HSPA2 expression suggests that it can influence the phenotype and survival of cancer cells similarly as overexpression of major members of the HSP70 gene family. Until now, neither the structure of the transcription unit of the human HSPA2 gene has been established nor a functional analysis of its promoter performed. In this study we established that the human HSPA2 gene, in contrast to its rodent counterparts, is intronless and has a single transcription start site. We also show that the same type of HSPA2 transcripts are synthesized in the testes and in cancer cell lines. In order to perform a functional study of the HSPA2 promoter, we used a transient transfection assay and found that the 392 bp fragment upstream of the ATG codon was a minimal region required for efficient transcription, while a 150 bp deletion from the 5' end of this region dramatically reduced the promoter activity. Delineation of the minimal promoter is a basic step toward identifiying the cis and trans elements involved in the regulation of the HSPA2 gene expression in cancer cells.

3

Construction of cDNA library from liver RNA of heat shocked rats and DNA sequence analysis of the clone containing the 3' -end untranslated region (3'UTR) of the heat inducible gene hsp70.2

82%

Lisowska K., Ścieglińska D., Krawczyk Z.

Acta Biochimica Polonica

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1996

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vol. 43

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issue 2

313-317

4

Functional analysis of spermatocyte-specific hst70 gene promoter in transgenic mice

82%

Widłak W., Markkula M., Krawczyk Z., Huhtaniemi I.

Acta Biochimica Polonica

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1994

62%

Widłak W., Vydra N., Dudaladava V., Ścieglińska D., Winiarski B., Krawczyk Z.

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2007

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vol. 54

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issue 1

107-112

EN

The Hsp70.2/Hst70 gene, which belongs to the 70 kDa heat-shock protein (HSP) family, is expressed specifically in primary spermatocytes and spermatids. The regulatory elements required for a high level of testis-specific expression of the gene are placed between the two major transcription start sites T1 and T2 (approximately 350 and 115 bp upstream of the starting ATG codon). Here we have shown that sequences proximal to the exon1/intron splicing site in the 5' untranslated region of the Hsp70.2/Hst70 gene, which include a highly conserved element called box B, are required for efficient expression of the chloramphenicol acetyltransferase reporter gene in testes of transgenic mice. However, in spite of the drastically reduced overall activity, the stage-specific expression pattern of the transgene was preserved after removal of these sequences. We have also shown that GC-box located downstream of the box B (approximately 210 bp upstream of the starting ATG codon) is indispensable for efficient expression of the Hsp70.2/Hst70 gene promoter in spermatogenic cells. The GC-box specifically binds proteins present in nuclear extracts from testes (putatively Sp1-like factors). A change in the pattern of such GC-box-interacting factors corresponds to activation of the Hsp70.2/Hst70 gene, confirming the importance of this regulatory element.

11

Synthetic derivatives of genistein, their properties and possible applications

62%

Rusin A., Krawczyk Z., Grynkiewicz G., Gogler A., Zawisza-Puchałka J., Szeja W.

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2010

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vol. 57

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issue 1

23-34

EN

Genistein, the principal isoflavone constituent of soybean, attracts much attention as a natural molecule with significant affinity towards targets of potential medicinal interest, but also as a food supplement or prospective chemopreventive agent. Since its physicochemical properties are considered suboptimal for drug development, much effort has been invested in designing its analogs and conjugates in hope to obtain compounds with improved efficacy and selectivity. The aim of this article is to summarize current knowledge about the properties of synthetic genistein derivatives and to discuss possible clinical application of selected novel compounds. Some basic information concerning chemical reactivity of genistein, relevant to the synthesis of its derivatives, is also presented.

12

Expression of a constitutively active mutant of heat shock factor 1 under the control of testis-specific hst70 gene promoter in transgenic mice induces degeneration of seminiferous epithelium.

52%

Widłak W., Benedyk K., Vydra N., Głowala M., Ścieglińska D., Małusecka E., Nakai A., Krawczyk Z.

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2003

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vol. 50

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issue 2

535-541

EN

Heat shock activates in somatic cells a set of genes encoding heat shock proteins which function as molecular chaperones. The basic mechanism by which these genes are activated is the interaction of the specific transcription factor HSF1 with a regulatory DNA sequence called heat shock element (HSE). In higher eukaryotes HSF1 is present in unstressed cells as inactive monomers which, in response to cellular stress, aggregate into transcriptionally competent homotrimers. In the present paper we showed that the expression of a transgene encoding mutated constitutively active HSF1 placed under the control of a spermatocyte-specific promoter derived from the hst70 gene severely affects spermatogenesis. We found the testes of transgenic mice to be significantly smaller than those of wild-type males and histological analysis showed massive degeneration of the seminiferous epithelium. The lumen of tubules was devoid of spermatids and spermatozoa and using the TUNEL method we demonstrated a high rate of spermatocyte apoptosis. The molecular mechanism by which constitutively active HSF1 arrests spermatogenesis is not known so far. One can assume that HSF1 can either induce or repress so far unknown target genes involved in germ cell apoptosis.

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Inhibition of RNA polymerase B activity in normal and regenerating rat liver after administration of 9-aminoacridine

The structural and functional analysis of the human HSPA2 gene promoter region

Construction of cDNA library from liver RNA of heat shocked rats and DNA sequence analysis of the clone containing the 3' -end untranslated region (3'UTR) of the heat inducible gene hsp70.2

Functional analysis of spermatocyte-specific hst70 gene promoter in transgenic mice

A rat testis-specific hsp70 gene-related transcript is coded by a novel gene from the hsp70 multigene family

Changes in cellular concentration of DNA-dependent RNA polymerases A and B during regeneration of rat liver

Identification of a microsatellite region composed of a long homopurine/homopyrimidine tract surrounded by AT -rich sequences upstream of the rat stress-inducible hsp70.1 gene

Isolation and structural analysis of rat heat-inducible hsp70 gene

Isolation of gene coding for major heat shock protein HSP71 in rat (wykreślone)

The GC-box is critical for high level expression of the testis-specific Hsp70.2/Hst70 gene

Synthetic derivatives of genistein, their properties and possible applications

Expression of a constitutively active mutant of heat shock factor 1 under the control of testis-specific hst70 gene promoter in transgenic mice induces degeneration of seminiferous epithelium.