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Associations between the prolactin receptor gene polymorphism and reproductive traits of boars

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Kmiec M., Terman A.

Journal of Applied Genetics

2006

vol. 47

issue 2

139-141

The prolactin receptor gene (PRLR), located on chromosome 16 in pigs, is a candidate gene for reproductive traits. The experiment was aimed to detect the DNA mutations in this gene and to find probable relations between the genotype and some reproductive traits in boars. The polymorphism in the PRLR gene was identified by PCR-RFLP method using specific primers and the restriction enzyme AluI. In total 229 boars of various breeds were genotyped. The frequency of allele A was estimated at 0.62 and allele B at 0.38. Genotype AA was found at a frequency of 0.45, AB at 0.35 and BB at 0.20. We found associations between PRLR genotype and ejaculate volume, sperm concentration, percentage of live sperm, and number of live sperm in the ejaculate (P < 0.01).

Single nucleotide polymorphisms in exon 10 of the chinchilla growth hormone receptor (GHR) gene

81%

Polasik D., Kmiec M., Liefers S., Terman A.

Journal of Applied Genetics

2005

vol. 46

issue 4

403-406

The aim of this study was to detect SNPs in exon 10 of the chinchilla growth hormone receptor gene (GHR) by comparative sequencing. Sixty females of the same breed (Standard) were analysed. Four new SNPs were identified, which cause 3 amino acid substitutions in the intracellular domain of the receptor: G/C at position 135 bp (in relation to the total sequence of exon 10) (gln/his), CAG/AAA at 352 bp and 354 bp (gln/lys), and C/A at 641 bp (thr/asn).

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2 Journal of Applied Genetics

1 2006

1 2005

2 Kmiec M.

2 Terman A.

1 Liefers S.

1 Polasik D.

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Associations between the prolactin receptor gene polymorphism and reproductive traits of boars

Single nucleotide polymorphisms in exon 10 of the chinchilla growth hormone receptor (GHR) gene