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Cytoprotective effect of silybin against lasalocid-induced toxicity in HepG2 cells

100%
Radko L., Cybulski W., Rzeski W.
Polish Journal of Veterinary Sciences
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2013
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issue 2
2

Gelatin sponge Spongostan as a carrier for animal and human adherent cell culture

88%
Paduch R., Rzeski W., Zdzisinska B., Kaczor J., Kandefer-Szerszen M.
Biotechnologia
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2003
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issue 1
223-233
EN
A porous gelatin sponge Spongostan (Ferrosan Denmark) as a carrier for the cultivation of several adherent cell lines and strains was tested. This material provide considerably greater surface available for cells than conventional bottles. Moreover, it allows to maintain high cell viability (over 87%) during culture time. When cells were cultured on Spongostan discs (wet vol. 0.11 cm3), no phenotypic changes in cell adherence and proliferation dynamics were observed. However, when cells adhered to Spongostan were frozen down and kept for 5 months in liquid nitrogen, they lost about 65% of their viability. Generally, Spongostan can be considered as a universal carrier for three-dimensional culture of many cell types, but it cannot be used for cells? freezing.
3
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Cell death and neuronal arborization upon quercetin treatment in rat neurons

88%
Jakubowicz-Gil J., Rzeski W., Zdzisinska B., Dobrowolski P., Gawron A.
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EN
Quercetin, one of the major flavonoids, exhibits many beneficial effects on human organism as antihistamine, antioxidant, anti-inflammatory, anticancer and antiviral drug. It is recommended as suplement of healthy diet but still the knowledge of its beneficial effect on normal cells is not satisfactory. We decided to examine the effect of flavonoid on neurons morphology and their susceptibility to cell death. Fractal analysis of rat neurons revealed that 24 hours long incubation with quercetin diminished neuronal arborisation in cortical neurons. Neurons also appeared to be very sensitive to cell death after flavonoid treatment in concentration dependent manner. Over 50% of cells died after incubation with 15 ?g/ml of flavonoid while 1 ?g/ ml of quercetin induced cell death only in 5%. Staining with Hoechst 33342 and propidium ioidide revealed the two types of cell death: apoptosis and necrosis. The number of apoptotic cells was comparable with necrotic ones. These results suggest toxic effect of quercetin on neurons what should be taken into consideration in further studies on using quercetin as therapeutic agent.
4
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Different sensitivity of neurons and neuroblastoma cells to quercetin treatment

76%
Jakubowicz-Gil J., Rzeski W., Zdzisinska B., Piersiak T., Weiksza K., Glowniak K., Gawron A.
Acta Neurobiologiae Experimentalis
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2008
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vol. 68
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issue 4
463-476
EN
determined the sensitivity of neurons and neuroblastoma cells on apoptosis and necrosis induction upon quercetin treatment. No expression of Hsp72 was observed in neurons, which were more sensitive to cell death upon quercetin treatment than neuroblastoma cells, where Hsp72 expression was observed. Reduction of Hsp72 gene expression in neuroblastoma cells by antisense oligonucleotides made them more sensitive to pro-apoptotic action of quercetin. Moreover, the flavonoid decreased Hsp27, procaspase-3, MRP and PKB expression in neuroblastoma cells and in neurons. Nuclear localization of mainly cytoplasmic Hsp27 was observed in neuroblastoma cells after treatment with high quercetin concentrations, while in neurons, the protein was present in nuclei both in control and quercetin treated cells. Our results suggest that quercetin induce apoptosis more effectively in cells with low level of Hsp 72 expression. Higher sensitivity of neurons for cell death after treatment with high quercetin concentrations in comparison to neuroblastoma cell line should also be taken into consideration in further studies on using studied flavonoid as therapeutic agent.
5

Propagation of Derzsy disease virus in goose embryo fibroblasts. An application of the Celligen Plus bioreactor

64%
Rzeski W., Paduch R., Kozdrun W., KaczorJ., Czekaj H., Samorek-Salamonowicz E., Kandefer-Szerszen M., Zdzisinska B., Dawidowicz A.
Biotechnologia
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2003
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issue 2
261-267
EN
Derzsy desease is a fatal hepatitis of young geese under the age of 5 weeks. The etiological agent is a goose parvovirus (GPV) which is widespread in all major goose farming countries in Europe and Asia. At present, vaccination is the only method preventing the loss of animals. In vitro GPV replicates only in goose embryo fibroblasts (GEF) and this way the virus is propagated in order to obtain the material for diagnostic tests and vaccine production. In this paper, we report the application of bioreactor Celligen Plus for GEF culture and Derzsy disease virus propagation. The method is based on immobilization and culture of cells on porous glass carriers in bioreactor. Comparing to the traditional cell culture techniques, the described method was over 12-fold more efficient and allowed to obtain good quality virus pool for Derzsy disease vaccine preparation.
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