Search results

1

Effect of gamma irradiation on blood plasma fibrinogen

100%

Krajewski T., Cierniewski C. S., University of Łódź I. o. B. a. B.

Acta Universitatis Lodziensis. Folia Biochimica et Biophysica

|

1983

|

vol. 2

2

The effect of anti-β_43-47 Fab fragments on expression of the polymerization sites in the E domain of fibrinogen

100%

Cierniewski C. S., Poniatowski J.

Acta Biochimica Polonica

|

1990

|

vol. 37

|

issue 1

31-37

3

Receptor TCR oraz białka współdziałające podczas aktywacji limfocytów T

99%

Cierniewski C. S.

Kosmos

|

2000

|

vol. 49

|

issue 3

481-487

4

Modification of fibrin network ultrastructure by Fab fragments specific for different domain of fibrinogen

81%

Cierniewski C. S., Janiak A., Wyroba E.

Acta Biochimica Polonica

|

1986

|

vol. 33

|

issue 3

195-202

5

The amino acids that constitute sequence γ268-282 of fibrinogen are not involved in fibrin monomer polymerization

81%

Janiak A., Plucinski T., Kupryszewski G., Cierniewski C. S.

|

vol. 40

|

issue 4

515-520

6

GATA-1 binding to the αV promoter negatively regulates expression of the integrin αV subunit in human leukemic K562 cells.

81%

Czyż M., Stasiak M., Boncela J., Cierniewski C. S.

|

EN

Recently we observed that the transcription factors Sp1 and Sp3 bind to the CTCCTCCTC sequence located between positions -194 and -172 of the αV promoter region and are directly involved in the regulation of transcriptional activity of the αV gene in human umbilical vascular endothelial cells (HUVECs) (Czyz & Cierniewski, 1999, Eur. J. Biochem. 265, 638). In this report we provide evidence that the GATA-1 factor regulates αV expression during differentiation of pluripotent K562 cells induced either by phorbol 12-myristate 13-acetate (PMA) or butyric acid (BA) through interaction with the GATA element in the αV gene promoter. DNase I footprinting analysis revealed that region -413 to -408, covering the GATA binding site, was protected by nuclear extract from K562 cells. There was no protection of this region by HUVEC nuclear extract. Electrophoretic mobility shift assay (EMSA) analysis of nuclear extract of K562 cells treated with BA revealed an increase in GATA binding activity, which was associated with reduced αV mRNA and αV protein on the cell surface. Stimulation of K562 cells with PMA resulted in opposite effects: lower expression of GATA-1 was associated with increased levels of αV. We conclude that the GATA-1 transcription factor specifically binds to the GATA element in the αV gene promoter and negatively regulates αV gene expression.

7

Interferon gamma bound to endothelial cells is phosphorylated by ecto-protein kinases

80%

Al-Nedawi K. N. I., Pawłowska Z., Cierniewski C. S.

Acta Biochimica Polonica

|

1999

|

vol. 46

|

issue 3

693-702

8

Corpora amylacea from multiple sclerosis brain tissue consists of aggregated neuronal cells

62%

Selmaj K., Pawłowska Z., Walczak A., Koziołkiewicz W., Raine C. S., Cierniewski C. S.

|

2008

|

vol. 55

|

issue 1

43-50

EN

In this report, we describe proteomic analysis of corpora amylacea collected by postmortem laser microdissection from multiple sclerosis (MS) brain lesions. Using low level protein loads (about 30 µg), a combination of two-dimensional electrophoresis with matrix-assisted laser desorption/ionization-time of flight mass spectrometry and database interrogations we identified 24 proteins of suspected neuronal origin. In addition to major cytoskeletal proteins like actin, tubulin, and vimentin, we identified a variety of proteins implicated specifically in cellular motility and plasticity (F-actin capping protein), regulation of apoptosis and senescence (tumor rejection antigen-1, heat shock proteins, valosin-containing protein, and ubiquitin-activating enzyme E1), and enzymatic pathways (glyceraldehyde-3-dehydrogenase, protein disulfide isomerase, protein disulfide isomerase related protein 5, lactate dehydrogenase). Samples taken from regions in the vicinity of corpora amylacea showed only traces of cellular proteins suggesting that these bodies may represent remnants of neuronal aggregates with highly polymerized cytoskeletal material. Our data provide evidence supporting the concept that biogenesis of corpora amylacea involves degeneration and aggregation of cells of neuronal origin.

9

The epidermal growth factor-like domain from tissue plasminogen activator. Cloning in E. coli, purification and ESR studies of its interaction with human blood platelets

52%

Pietrucha T., Stec W. J., Okruszek A., Uznański B., Koziołkiewicz M., Wilk A., Płucienniczak A., Świątkowska M., Cierniewski C. S.

Acta Biochimica Polonica

|

1994

|

vol. 41

|

issue 1

25-34

10

Synthesis, biochemical and biological studies on oligonucleotides bearinga lipophilic dimethoxytrityl group

52%

Misiura K., Wójcik M., Krakowiak A., Koziołkiewicz M., Pawłowska Z., Pluskota E., Cierniewski C. S., Stec W. J.

Acta Biochimica Polonica

|

1998

|

vol. 45

|

issue 1

27-32

11

Inhibition of plasminogen activator inhibitor release in endothelial cell cultures by antisense oligodeoxyribonucleotides with a 5˘-end lipophilic modification

42%

Kobylańska A., Pluskota E., Światkowska M., Wójcik M., Cierniewska-Cieślak A., Krakowiak A., Boczkowska M., Pawłowska Z., Okruszek A., Koziołkiewicz M., Cierniewski C. S., Stec W. J.

Acta Biochimica Polonica

|

1999

|

vol. 46

|

issue 3

679-691

Refine search results

Effect of gamma irradiation on blood plasma fibrinogen

The effect of anti-β_43-47 Fab fragments on expression of the polymerization sites in the E domain of fibrinogen

Receptor TCR oraz białka współdziałające podczas aktywacji limfocytów T

Modification of fibrin network ultrastructure by Fab fragments specific for different domain of fibrinogen

The amino acids that constitute sequence γ268-282 of fibrinogen are not involved in fibrin monomer polymerization

GATA-1 binding to the αV promoter negatively regulates expression of the integrin αV subunit in human leukemic K562 cells.

Interferon gamma bound to endothelial cells is phosphorylated by ecto-protein kinases

Corpora amylacea from multiple sclerosis brain tissue consists of aggregated neuronal cells

The epidermal growth factor-like domain from tissue plasminogen activator. Cloning in E. coli, purification and ESR studies of its interaction with human blood platelets

Synthesis, biochemical and biological studies on oligonucleotides bearinga lipophilic dimethoxytrityl group

Inhibition of plasminogen activator inhibitor release in endothelial cell cultures by antisense oligodeoxyribonucleotides with a 5˘-end lipophilic modification