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Promoting phorbol ester-TPA enhances migration of C3H 10TI/2ells.The role of protein kinase C and its relation to carcinogenesis

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Janik P., Szaniawska B., Kowalczyk D., Maternicka K.
Folia Biologica
|
1995
|
vol. 43
|
issue 3-4
83-87
EN
Treatment of 10T1/2 cells with promoting phorbol ester drasticly enhanced migration studied fibroblasts in a serum-supplemented medium.The same cells when exposed to ionomycin or TPA in a serum-free medium did not show any migration.The addition of 1% of serum induced spontaneous and TPA stimulated migration.Also EGF and PDGE separatly or together induced the migration of 10T1/2 cells.Parallel studies of protein kinase C documented low enzymatic activity after treatment with TPA, whereas transcripts of PKC were shown independently of TPA treatment.
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