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1

Production of L-lactic acid from whey by selected Lactobacillus strains

100%
Zlotkowska H., Czakaj J., Krakowiak A.
Biotechnologia
|
2004
|
issue 1
274-283
EN
From among of six strains of Lactobacillus able for production of lactic acid in whey medium, the strain of Lactobacillus delbrueckii sp. bulgaricus s/l was chosen for further experiments as most suitable one. The effect of inoculum concentration and the composition of the medium on the production of lactic acid were studied. It was found that for the inoculation of the medium, 10% (v/v) of the inoculum of propagation lasting 18 h, in the medium MRS containing lactose, should be applied. The amount of lactic acid obtained varied from 3,1 to 7,1 g/100 ml medium, depending on the composition of the medium. Produced L(+) lactic acid constituted over 95% of the sum of D and L isomers. When whey was supplemented with yeast autolisate, lactic acid production and yield process increased. The maximum yield of lactic acid production (97,3%) was obtained when L. delbrueckii sp. bulgaricus s/l was cultured for 44 h in the temperature of 43oC in medium containing whey enriched with 5% of yeast autolisate and 6% of CaCO3.
2

Intensification of lipase biosynthesis as a result of electrofusion of Rhizopus cohnii protoplasts

88%
Sawicka-Zukowska R., Juszczakiewicz D., Misiewicz A., Krakowiak A., Jedrychowska B.
Journal of Applied Genetics
|
2004
|
vol. 45
|
issue 1
37-48
EN
Our objective was to obtain products of fusion of the filamentous fungus Rhizopus cohnii Rh.c./1 with an increased capacity for lipase biosynthesis in comparison with the original strain. Protoplasts of auxotrophic mutants of the parent strain Rh.c./1 obtained after UV irradiation of the spores were subjected to electrofusion. We found that the largest number of electrofusion products could be obtained with the use of the following process parameters: 1 or 2 impulses immediately following one another with a field intensity of 200 V/cm and an exposition time of 1000 ms at the stage of dielectrophoresis, 1 impulse with a field intensity of 500 V/cm and an exposition time of 10 ms or 20 ms at the stage of fusion, regulated temperature of 4oC before and after the process, rounding time of ca 20 min. Electrofusion of protoplasts of auxotrophic mutants of the Rh.c./1 strain produced 19 fusion products whose lipase biosynthesis capacity in a liquid medium culture was higher than that of the parent strains. The fusion product labelled XIII-21 was selected as the best strain. Lipase activity obtained after its culture in the liquid medium was ca 3.5 times higher than that obtained after the culture of the original strain Rh.c./1.
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