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Microglial and astroglial cells in the rat paraclaustral reservoir during postnatal development: an immunohistochemical study

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Ludkiewicz B., Domaradzka-Pytel B., Morys J.

Acta Neurobiologiae Experimentalis

2001

vol. 61

issue 1

35-43

A immunohistochemical study of postnatal development of the paraclaustral reservoir of migrating cells in the rat brain was performed using anti-GFAP (for astroglia), ED1 and OX-42 (for microglia) antibodies. From birth to the 4th day of postnatal life most GFAP-positive cells in the paraclaustral reservoir are similar to transitional astroglia. From the end of the first postnatal week they have the morphology of mature astrocytes, although during the next week, their density was a slightly higher than in neighboring structures. On the 21st day, the morphology and density of astroglial cells in the ventral part of the external capsule did not differ from the surrounding regions. ED1/OX-42- positive microglial cells present in the paraclaustral reservoir during the first postnatal week represented ameboid microglia; their density was clearly higher than in the neighboring structures. During the second week they began to transform into ramified microglia and from the 21st day on, only OX-42 positive resting microglial cells were observed in the ventral part of the external capsule. We suggest that the paraclaustral reservoir is a place of accumulation of astroglia and microglia during brain development and may possibly serve as source of glial cells for neighboring structures. Alternatively, these glial populations may perform local developmental functions.

Analysis of calcium binding protein immunoreactivity in the claustrum and the endopiriform nucleus of the rabbit

76%

Wojcik S., Dziewiatkowski J., Spodnik E., Ludkiewicz B., Domaradzka-Pytel B., Kowianski P., Morys J.

Acta Neurobiologiae Experimentalis

2004

vol. 64

issue 4

449-460

The present paper describes parvalbumin, calbindin-D28k and calretinin immunoreactivity in the claustrum and endopiriform nucleus of adult rabbits. Studied neuronal populations are characterized by morphological heterogeneity. Four types were identified in each subpopulation of cells containing calcium binding proteins on the basis of the number of processes and their branching pattern. There were no spatial differences in the distribution of cells containing either parvalbumin or calbindin-D28k in the claustrum and endopiriform nucleus. Well documented presence of the various projective zones in the rabbit claustrum did not reflect the specific distribution of neurons containing calcium binding proteins, except those containing calretinin. Their localization may correspond with the limbic zone. We have found that the rabbit claustrum and endopiriform nucleus have different pattern of parvalbumin and calretinin immunoreactivity. The former was more intense in the claustrum and the distribution of cell types was significantly different from that in the endopiriform nucleus. Calretinin-positive cells were observed in the claustrum, while in the endopiriform nucleus they were scarce. The distribution of neither calbindin-D28k-ir neurons nor fibers allowed differentiation of claustrum and endopiriform nucleus. Significant differences between the claustrum and endopiriform nucleus observed in the rabbit might be related with ontogenetic as well as other (functional) factors.

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2 Acta Neurobiologiae Experimentalis

1 2004

1 2001

2 Domaradzka-Pytel B.

2 Ludkiewicz B.

2 Morys J.

1 Dziewiatkowski J.

1 Kowianski P.

1 Spodnik E.

1 Wojcik S.

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Microglial and astroglial cells in the rat paraclaustral reservoir during postnatal development: an immunohistochemical study

Analysis of calcium binding protein immunoreactivity in the claustrum and the endopiriform nucleus of the rabbit