Search results

1

The double helix of DNA

100%

Barciszewski J.

Biotechnologia

|

1993

|

issue 3

138-144

EN

Short history of the path to the of was described. Special attention was been paid to the early work of Rosalin Franclin, Maurice Wilkins and Erwin Charagaff. Also some general consequences of the for biological sciences were discused.

2

Methionyl-tRNA synthetase.

64%

Deniziak M. A., Barciszewski J.

|

2001

|

vol. 48

|

issue 2

337-350

EN

Methionyl-tRNA synthetase (MetRS) belongs to the family of 20 enzymes essential for protein biosynthesis. It links covalently methionine with its cognate tRNA. Crystal structures solved for bacterial MetRSs have given a number of interesting insights into enzyme architecture and methionylation catalysis. A comparison of sequences of MetRSs belonging to all kingdoms of life, as well as numerous biochemical and genetic studies have revealed the presence of various additional domains appended to the catalytic core of synthetase. They are responsible for interactions with tRNA and proteins. Tertiary structure of C-terminal tRNA-binding appendices can be deduced from those determined for their homologues: tRNA binding protein 111 and endothelial monocyte-activating polypeptide II. Contacts between MetRS and other proteins could be mediated not only by noncatalytic peptides but also by structural elements present in the catalytic core, e.g. Arg-Gly-Asp (RGD) motifs. Additional activities involve MetRS in the maintenance of translational fidelity and in coordination of ribosome biogenesis with protein synthesis.

3

Understanding evolution

64%

Barciszewski J., Legocki A. B.

|

2001

|

vol. 48

|

issue 2

291-294

4

The genetic code - 40 years on

64%

Szymański M., Barciszewski J.

|

2007

|

vol. 54

|

issue 1

51-54

EN

The genetic code discovered 40 years ago, consists of 64 triplets (codons) of nucleotides. The genetic code is almost universal. The same codons are assigned to the same amino acids and to the same START and STOP signals in the vast majority of genes in animals, plants, and microorganisms. Each codon encodes for one of the 20 amino acids used in the synthesis of proteins. That produces some redundancy in the code and most of the amino acids being encoded by more than one codon. The two cases have been found where selenocysteine or pyrrolysine, that are not one of the standard 20 is inserted by a tRNA into the growing polypeptide.

5

Selected small molecules as inducers of pluripotency

52%

Baranek M., Markiewicz W., Barciszewski J.

|

2016

|

vol. 63

|

issue 4

709-716

EN

The general idea of regenerative medicine is to fix or replace tissues or organs with live and patient-specific implants. Pluripotent stem cells are capable of indefinite self-renewal and differentiation into all cell types of the body. An easily accessible source of induced pluripotent stem cells (iPSCs) may allow obtaining and culturing tissues in vitro. Many approaches in the methods leading to obtain iPSCs have been tested in order to limit immunogenicity and tumorigenesis, and to increase efficiency. One of the approaches causing pluripotency is usage of small molecule compounds. It would be of great importance to assess their specific properties and reveal their new capacity to induce pluripotent stem cells and to improve reprogramming efficiency. Identification of the epigenetic changes during cellular reprogramming will extend our understanding of stem cell biology and many therapeutic applications. In this paper we discuss mainly the nucleotide derivatives, already proven or for now only putative inducers of the cells' pluripotency, that modulate the epigenetic status of the cell.

6

The ribonuclease activity of the two synthetic polypeptides having zinc finger sequence

52%

Giel M., Rekowski P., Kupryszewski G., Barciszewski J.

|

vol. 40

|

issue 1

32-34

7

Regulatory RNAs in Planarians

52%

Pawlicka K., Perrigue P., Barciszewski J.

|

2016

|

vol. 63

|

issue 4

681-686

EN

The full scope of regulatory RNA evolution and function in epigenetic processes is still not well understood. The development of planarian flatworms to be used as a simple model organism for research has shown a great potential to address gaps in the knowledge in this field of study. The genomes of planarians encode a wide array of regulatory RNAs that function in gene regulation. Here, we review planarians as a suitable model organism for the identification and function of regulatory RNAs.

8

Structure and functions of 5S rRNA.

52%

Barciszewska M. Z., Szymański M., Erdmann V. A., Barciszewski J.

|

2001

|

vol. 48

|

issue 1

191-198

EN

The ribosome is a macromolecular assembly that is responsible for protein biosynthesis in all organisms. It is composed of two-subunit, ribonucleoprotein particles that translate the genetic material into an encoded polypeptides. The small subunit is the site of codon-anticodon interaction between the messenger RNA (mRNA) and transfer RNA (tRNA) substrates, and the large subunit catalyses peptide bond formation. The peptidyltransferase activity is fulfilled by 23S rRNA, which means that ribosome is a ribozyme. 5S rRNA is a conserved component of the large ribosomal subunit that is thought to enhance protein synthesis by stabilizing ribosome structure. This paper shortly summarises new results obtained on the structure and function of 5S rRNA.

9

The nucleotide sequence of ribosomal 5S RNA from Rhizobium meliloti: comparision with 5S RNA of Agrobacterium

52%

Nalasowska M., Barciszewska M., Barciszewski J.

Acta Biochimica Polonica

|

1989

|

vol. 36

|

issue 3-4

303-309

10

Evidence for plant ribosomal 58 RNA involvement in elongation of polypeptide chain biosynthesis

52%

Shaikhin S., Sobkiewicz A., Barciszewski J., Twardowski T.

Acta Biochimica Polonica

|

1994

|

vol. 41

|

issue 1

57-62

11

The new aspects of aminoacyl-tRNA synthetases.

52%

Szymański M., Deniziak M., Barciszewski J.

|

EN

Aminoacyl-tRNA synthetases (AARS) are essential proteins found in all living organisms. They form a diverse group of enzymes that ensure the fidelity of transfer of genetic information from the DNA into the protein. AARS catalyse the attachment of amino acids to transfer RNAs and thereby establish the rules of the genetic code by virtue of matching the nucleotide triplet of the anticodon with its cognate amino acid. Here we summarise the effects of recent studies on this interesting family of multifunctional enzymes.

12

RNA dactyloscopy

52%

Gabryelska M., Wyszko E., Barciszewski J.

|

2016

|

vol. 63

|

issue 4

785-787

EN

Despite the wealth of data on RNA secondary structure, conformational dynamics and tertiary structure in vitro and in vivo, predicting RNA biological activity in cellular environments remains difficult. Here, we present a comparison between in silico RNA fingerprinting and published experimental data that sheds light on efficient design of the hammerhead ribozyme molecules with a high intracellular efficiency. Our method, which we call RNA dactyloscopy, is a reliable tool for assessing the catalytic properties, modeling and design of RNA.

13

The calculation of plant 5S rRNAs secondary structure

45%

Joachimiak A., Nalaskowska M., Barciszewska M. Z., Mashkova T. D., Barciszewski J.

Acta Biochimica Polonica

|

1989

|

vol. 36

|

issue 3-4

215-223

14

Tertiary structure and computer modeling of plant 5S ribosomal RNA

45%

Joachimiak A., Basavappa R., Barciszewska M. Z., Nalaskowska M., Barciszewski J.

Acta Biochimica Polonica

|

1990

|

vol. 37

|

issue 3

359-375

15

The decisive role of the water structure in changes of conformation of nucleic acids

45%

Barciszewski J., Jurczak J., Porowski S., Specht T., Erdmann V. A.

Acta Biochimica Polonica

|

1999

|

vol. 46

|

issue 1

133-144

16

Rapid separation of tyrosine-specific tRNA from white lupin

45%

Barciszewska M. Z., Krajewski J., Gawrońska I., Barciszewski J.

Acta Biochimica Polonica

|

1992

|

vol. 39

|

issue 2

223-226

17

Cloning and sequencing of cDNA encoding the rice methionyl-tRNA synthetase

45%

Deniziak M., Mirande M., Barciszewski J.

Acta Biochimica Polonica

|

1998

|

vol. 45

|

issue 3

669-676

18

Quantification of 5-methyl-2'-deoxycytidine in the DNA

39%

Giel-Pietraszuk M., Insińska-Rak M., Golczak A., Sikorski M., Barciszewska M., Barciszewski J.

|

2015

|

vol. 62

|

issue 2

281-286

EN

Methylation at position 5 of cytosine (Cyt) at the CpG sequences leading to formation of 5-methyl-cytosine (m5Cyt) is an important element of epigenetic regulation of gene expression. Modification of the normal methylation pattern, unique to each organism, leads to the development of pathological processes and diseases, including cancer. Therefore, quantification of the DNA methylation and analysis of changes in the methylation pattern is very important from a practical point of view and can be used for diagnostic purposes, as well as monitoring of the treatment progress. In this paper we present a new method for quantification of 5-methyl-2'deoxycytidine (m5C) in the DNA. The technique is based on conversion of m5C into fluorescent 3,N4-etheno-5-methyl-2'deoxycytidine (εm5C) and its identification by reversed-phase high-performance liquid chromatography (RP-HPLC). The assay was used to evaluate m5C concentration in DNA of calf thymus and peripheral blood of cows bred under different conditions. This approach can be applied for measuring of 5-methylcytosine in cellular DNA from different cells and tissues.

19

Interaction of HIV Tat model peptides with tRNA and 5S rRNA

39%

Giel-Pietraszuk M., Barciszewska M. Z., Mucha P., Rekowski P., Kupryszewski G., Barciszewski J.

Acta Biochimica Polonica

|

1997

|

vol. 44

|

issue 3

591-600

20

A-Z conformational changes effected in RNA by high pressure

39%

Krzyżaniak A., Fürste J. P., Sałański P., Jurczak J., Erdmann V. A., Barciszewski J.

|

vol. 40

|

issue 1

66-68

Refine search results

The double helix of DNA

Methionyl-tRNA synthetase.

Understanding evolution

The genetic code - 40 years on

Selected small molecules as inducers of pluripotency

The ribonuclease activity of the two synthetic polypeptides having zinc finger sequence

Regulatory RNAs in Planarians

Structure and functions of 5S rRNA.

The nucleotide sequence of ribosomal 5S RNA from Rhizobium meliloti: comparision with 5S RNA of Agrobacterium

Evidence for plant ribosomal 58 RNA involvement in elongation of polypeptide chain biosynthesis

The new aspects of aminoacyl-tRNA synthetases.

RNA dactyloscopy

The calculation of plant 5S rRNAs secondary structure

Tertiary structure and computer modeling of plant 5S ribosomal RNA

The decisive role of the water structure in changes of conformation of nucleic acids

Rapid separation of tyrosine-specific tRNA from white lupin

Cloning and sequencing of cDNA encoding the rice methionyl-tRNA synthetase

Quantification of 5-methyl-2'-deoxycytidine in the DNA

Interaction of HIV Tat model peptides with tRNA and 5S rRNA

A-Z conformational changes effected in RNA by high pressure