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1

Neutrophile activation by bacterial endotoxins

100%
Klink M., Kaca W.
Postępy Higieny i Medycyny Doświadczalnej
|
1996
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vol. 50
|
issue 4
333-350
EN
The endotoxin (lipopolysaccharide, LPS), major component of Gram-negative bacteria cell wall, activate numerous types of cells, neutrophile included. The chemical compositions of polysaccharide (O-specific polysaccharide and core oligosaccharide) and hydrophobic lipid A parts of LPS are presented. The bindings of LPSs to neutrophiles resulted in signal transduction and neutrophiles activation. Neutrophiles, under LPS stimulation, generate oxygen radicals, nitric oxide and other components of inflammatory processes.
2

Effect of exogenous opioid peptides on TNF--induced human neutrophil apoptosis in vitro

81%
Sulowska Z., Majewska E., Tchorzewski H., Klink M.
Archivum Immunologiae et Therapiae Experimentalis
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2003
|
vol. 51
|
issue 4
267-272
EN
Polymorphonuclear leukocytes (neutrophils) apoptosis is an important mechanism regulating the life span and some functions of neutrophils at inflamed sites. The opioid peptides are present in the peripheral circulation and their concentrations rapidly increase as the result of stress and inflammation. The effect of opioid peptides such as met-enkephalin (M-ENK) and beta-endorphin (beta-END) on tumor necrosis factor alpha (TNF-alpha)-induced apoptosis in human neutrophils in vitro was investigated. Neutrophils isolated from peripheral blood were cultured in the absence or presence of 106-1010 M of opioid peptides for 8, 12 and 18 hours. Features of apoptotic neutrophils were measured by flow cytometric method based on analysis of apoptotic nuclei (DNA content). We found that M-ENK and beta-END enhanced both non-induced and TNF-alpha-induced neutrophil apoptosis in vitro in a dose-dependent manner. The effect of opioid peptides on modulation of neutrophil apoptosis was not reversed by opioid-receptor antagonist naloxone. The results suggest that M-ENK and beta-END can regulate neutrophil life span via apoptosis and in this way may participate in resolution of inflammation.
3

Feto-maternal immunoregulation

81%
Klink M., Rozalska B., Rudnicka W.
Postępy Higieny i Medycyny Doświadczalnej
|
1994
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vol. 48
|
issue 5
543-563
EN
One of the great mysteries in modern immunology is how extraembryonic membranes escape rejection by maternal immune response, although they express paternal genes/antigenes which should stimulate allogenic recognition and rejection.Generally, two theories try to explain the pregnancy phenomenon.One of them emphasizes the role of immunosuppresive reactions in the protection of the fetus.On the contrary, the "immunotropism" theory insist on the importance of mother's immune antigens of the conceptus.Moreover, the last years abounded in discoveries on molecules regulating cell-cell interaction at the level of the initiation and effect or stage of the immune response.The best examples of such molecules could be extracellular matrix proteins, integrins, interleukins and various growth factors.The discussion of those molecules as regards their role in the protection of the fetus was the main aim of this article.
4

Serological differentiation of H. pylori CagA(+) and CagA(-) infections

52%
Chmiela M., Wisniewska M., Bak-Romaniszyn L., Rechcinski T., Planeta-Malecka I., Bielanski W., Konturek S. J., Plonka M., Klink M., Rudnicka W.
Archivum Immunologiae et Therapiae Experimentalis
|
2003
|
vol. 51
|
issue 2
131-136
EN
Many of H. pylori strains causing gastroduodenal diseases have a cagA gene encoding CagA protein, a virulence factor of these bacteria. Anti-CagA antibodies produced by majority of people infected with CagA(+) strains can indicate such infection. In this study the efficacy of three immunoenzymatic tests: immunoblot (MileniaID Blot H. pylori IgG, DPC Biermann GmbH, Germany) (MB) and ELISA, conducted with a recombinant immunodominant fragment of CagA (rCagA) and full length CagA molecule (flCagA), in detecting CagA(+) and CagA(-) infections, was compared. The 13C urea breath test (13C-UBT) was used for establishing H. pylori status. The serum samples from 157 individuals were used for serodiagnosis. The H. pylori CagA(+) infection was detected in H. pylori infected individuals with similar frequency by MB (64%) and flCagA-ELISA (60%) and little less frequently by rCagA-ELISA (53%). There was a high coincidence between the negative results of these three tests for H. pylori uninfected individuals with no anti-CagA IgG in the serum (96-100%). The results show that rCagA-ELISA and especially flCagA-ELISA are easy, inexpensive and useful noninvasive assays for discrimination of CagA(+) and CagA(-) H. pylori infections in the subjects examined by urea breath test.
5

Lack of relationship between serum content of MBL, sCD14, anti-PPD and anti-Hsp65 IgG, and ingestion of Mycobacterium bovis BCG bacilli by phagocytes

52%
Paziak-Domanska B., Bonar A., Kowalewicz-Kulbat M., Klink M., Kowalski M., Karhukorpi J., Karttunen R., Jurkiewicz M., Rozalska B., Rudnicka W.
Archivum Immunologiae et Therapiae Experimentalis
|
2002
|
vol. 50
|
issue 5
337-344
EN
Prophylactic vaccination against tuberculosis (TB) with a live attenuated strain of Mycobacterium bovis Bacille Calmett?e-Gerin (BCG) has been used worldwide. However, TB remains one of the most significant diseases of humans and animals. Better understanding of the mechanisms of human immunity to mycobacteria is essential for development of new vaccines and estimation of their efficacy. In this study we determined the levels of known humoral mediators of mycobacterial phagocytosis - mannose binding lectin (MBL), soluble CD14 (sCD14), antibodies of IgG class against mycobacterial purified protein derivative (PPD) and mycobacterial Hsp65 antigen, in the sera from healthy young volunteers vaccinated with BCG and presenting positive and negative Mantoux responses to PPD. Than we asked a question as to whether macrophages and polymorphonuclear leukocytes (PMNs) from the individuals with positive (TT(+)) and negative (TT(-)) tuberculin tests differ by the ability to ingest mycobacteria. Also we were looking for a relation between the intensity of mycobacterial ingestion by phagocytes in the medium with autologous sera containing different concentration of MBL, sCD14 andf anti-mycobacterial IgG. We found no significant differences between the investigated parameters for TT(+) and TT(-) volunteers. Our result suggest that ability of macrophages and PMNs to ingest mycobacteria depends on an individual intrinsic capacity of phagocytes.
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