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Zinc determination in extracts from bacopa monnieri shoot cultures into simulated gastric fluids.

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MUSZYŃSKA B., ROJOWSKI J., ŁOJEWSKI M., DZIWULSKA K., OPOKA W.

Medicina Internacia Revuo

2015

vol. 26

issue 105

175-179

Bacopa monnieri (Water hyssop) is a hydrophyte occurring in Asia and the southern US states. This plant is known in India as Brahmi and has been used in Ayurvedic medicine for 5,000 years as sedative, supporting the treatment of insomnia and improving cerebral circulation. For the biological effect in this species are responsible triterpenoids saponins – bacosides. The aim of the study was to obtain the biomass of B. monnieri shoot in vitro cultures, and then evaluating the biomass growth and content of zinc. B. monnieri derived from in vitro culture on Murashige-Skoog medium (MS) and on the same medium, but enriched in organic additives and elements may be a good source of Zinc for the human body. The experiment was also conducted on the commercial formulations containing B. monnieri. For the determination of zinc ions DP ASV method was used. It was found that the use of highly mineralized water (mineral water “Stefan” – Spa Szczawnica) for extraction of B. monnieri biomass from in vitro culture provided the highest concentration of zinc in the material and for the biomass from MS medium with the addition of As (163.52, and 167.23 mg/mL, respectively). The lowest content was found in B. monnieri biomass from MS medium supplemented with serine, and the capsules product (13.54 and 14.57 mg/mL). For extracts of B. monnieri biomass from MS medium enriched with Zn salts into digestive juices the highest concentration of zinc was determined in gastric juice (6.88 mg/mL).

EDIBLE MUSHROOMS IN PROPHYLAXIS AND TREATMENT OF HUMAN DISEASES

86%

MUSZYNSKA B., SUŁKOWSKA-ZIAJA K., ŁOJEWSKI M., OPOKA W., ZAJĄC M., ROJOWSKI J.

Medicina Internacia Revuo

2013

vol. 25

issue 101

170-183

In recent years an increase in the consumption of edible mushrooms has been observed. In many countries mushrooms have been a popular delicacy, as they add flavor and texture to a meal. Mushrooms are able to accumulate both primary and secondary metabolites. Some of them may play an antioxidant role, e.g. phenolic and indole compounds, flavonoids, terpenoids, sterols, ascorbic acid, ergothioneine and carotenoids and are a source of elements, e.g. selenium. Indole compounds fulfill the role of neurotransmitters or their precursors, exhibit antioxidant, anticancer, anti-inflammatory and anti-aging actions, regulate the diurnal cycle in humans and take part in blood coagulation. Biologically and therapeutically active metabolites of fungi are used to treat such serious diseases as cardiovascular diseases, diabetes, atherosclerosis and cancer. The intake of mushrooms clearly has a cholesterol-lowering effect or hypocholesterolemic effect by different mechanisms such as decreasing VLDL, improving lipid metabolism, inhibiting of activity of HMG-CoA reductase, and consequently preventing the development of atherosclerosis. The antioxidant and anti-inflammatory compounds occurring in mushrooms also may contribute to reduce the atherosclerosis risk.

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2 Medicina Internacia Revuo

1 2015

1 2013

2 OPOKA W.

2 ROJOWSKI J.

2 ŁOJEWSKI M.

1 DZIWULSKA K.

1 MUSZYNSKA B.

1 MUSZYŃSKA B.

1 SUŁKOWSKA-ZIAJA K.

1 ZAJĄC M.

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Zinc determination in extracts from bacopa monnieri shoot cultures into simulated gastric fluids.

EDIBLE MUSHROOMS IN PROPHYLAXIS AND TREATMENT OF HUMAN DISEASES