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Was the serine protease cathepsin G discovered by S. G. Hedin in 1903 in bovine spleen?

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Palesch D., Sieńczyk M., Oleksyszyn J., Reich M., Wieczerzak E., Boehm B. O., Burster T.

2011

vol. 58

issue 1

39-44

In the beginning of the 20th century, enzymes with proteolytic activity were classified as peptidases, Erepsin, and proteases. Among these, pepsin, trypsin, and autolytic enzymes were of the protease class. Spleen-derived proteases were poorly characterized until Sven Gustaf Hedin performed several digestion experiments with bovine spleen. He incubated minced bovine spleen under acidic or neutral conditions and characterized two active proteases; the results were published in 1903. The first protease was named α-protease and was active under neutral conditions. The second was named β-protease and was active under acidic conditions. We replicated Hedin's experiments according to his methods and found, by using activity-based probes to visualize proteases, that the historical α-protease is the present-day serine protease cathepsin G (CatG), which is known to be important in several immune processes, including antigen processing, chemotaxis, and activation of surface receptors. The β-protease, however, comprised different proteases including CatX, B, S, and D. We suggest that Hedin described CatG activity in bovine spleen over 100 years ago.

Differential effects of various soy isoflavone dietary supplements (nutraceuticals) on bacterial growth and human fibroblast viability

100%

Pierzynowska K., Rzeszótko A., Blendowska A., Wieczerzak E., Rodziewicz-Motowidło S., Piotrowska E., Węgrzyn G.

2018

vol. 65

issue 2

325-332

Flavonoids, polyphenolic compounds present in many food products, affect growth of different bacterial species when tested as purified or synthetic substances. They can also influence gene expression in human cells, like fibroblasts. Here, we asked if soy isoflavone extracts, commonly used in many products sold as anti-menopausal dietary supplements, influence bacterial growth similarly to a synthetic isoflavone, genistein. Four commercially available products were tested in amounts corresponding to genistein concentrations causing inhibition of growth of Vibrio harveyi (a model bacterium sensitive to this isoflavone) and Escherichia coli (a model bacterium resistant to genistein). Differential effects of various extracts on V. harveyi and E. coli growth, from stimulation, to no changes, to inhibition, were observed. Moreover, contrary to genistein, the tested extracts caused a decrease (to different extent) in viability of human dermal fibroblasts. These results indicate that effects of various soy isoflavone extracts on bacterial growth and viability of human cells are different, despite similar declared composition of the commercially available products.

Structural studies of cysteine proteases and their inhibitors.

68%

Grzonka Z., Jankowska E., Kasprzykowski F., Kasprzykowska R., Łankiewicz L., Wiczk W., Wieczerzak E., Ciarkowski J., Drabik P., Janowski R., Kozak M., Jaskólski M., Grubb A.

2001

vol. 48

issue 1

1-20

Cysteine proteases (CPs) are responsible for many biochemical processes occurring in living organisms and they have been implicated in the development and progression of several diseases that involve abnormal protein turnover. The activity of CPs is regulated among others by their specific inhibitors: cystatins. The main aim of this review is to discuss the structure-activity relationships of cysteine proteases and cystatins, as well as of some synthetic inhibitors of cysteine proteases structurally based on the binding fragments of cystatins.

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3 Acta Biochimica Polonica

1 2018

1 2011

1 2001

3 Wieczerzak E.

1 Blendowska A.

1 Boehm B. O.

1 Burster T.

1 Ciarkowski J.

1 Drabik P.

1 Grubb A.

1 Grzonka Z.

1 Jankowska E.

1 Janowski R.

1 Jaskólski M.

1 Kasprzykowska R.

1 Kasprzykowski F.

1 Kozak M.

1 Oleksyszyn J.

1 Palesch D.

1 Pierzynowska K.

1 Piotrowska E.

1 Reich M.

1 Rodziewicz-Motowidło S.

1 Rzeszótko A.

1 Sieńczyk M.

1 Wiczk W.

1 Węgrzyn G.

1 Łankiewicz L.

Search results

Was the serine protease cathepsin G discovered by S. G. Hedin in 1903 in bovine spleen?

Differential effects of various soy isoflavone dietary supplements (nutraceuticals) on bacterial growth and human fibroblast viability

Structural studies of cysteine proteases and their inhibitors.