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1

Interval mapping of QTLs controlling yield-related traits and seed protein content in Pisum sativum

100%
Irzykowska L., Wolko B.
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issue 3
297- 306
EN
A linkage map of garden pea was constructed on the basis of 114 plants (F2 generation) derived from a cross combination Wt10245 ? Wt11238. The map, consisting of 204 morphological, isozyme, AFLP, ISSR, STS, CAPS and RAPD markers, was used for interval mapping of quantitative trait loci (QTLs) controlling seed number, pod number, 1000-seed weight, 1000-yield, and seed protein content. Characterization of each QTL included identification of QTL position with reference to the flanking markers, estimation of the part of variance explained by this QTL, and determination of its gene action. The yield-related traits were measured in F2 plants and in F4 recombinant inbred lines (RILs). The interval mapping revealed two to six QTLs per trait, demonstrating linkage to seven pea chromosomes. A total of 37 detected QTLs accounted for 9.1-55.9% of the trait's phenotypic variation and showed different types of gene action. As many as eight and ten QTLs influencing the analysed traits were mapped in linkage groups III and V, respectively, indicating an important role of these regions of the pea genome in the control of yield and seed protein content.
2

Isozyme and RAPD markers for the identification of pea, field bean and lupin cultivars

81%
Wolko B., Swiecicki W. K., Kruszka K., Irzykowska L.
Journal of Applied Genetics
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2000
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vol. 41
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issue 3
151-165
EN
The limited gene pool used in breeding decreases the level of genetic diversity in legume cultivars. Morphological or physiological characters are not always sufficient for quick, easy and precise cultivar description. Isozyme variability of 33 commercial Polish pea cultivars was analysed. The level of allozyme polymorphism discovered was high enough for the identification of all cultivars within two groups: white flowering peas for human consumption and colored flowering peas for fodder. The range of RAPD marker polymorphism among three lupin crops (white lupin, narrow-leafed lupin and yellow lupin) was tested. It was possible to identify each of four white lupin cultivars by means of bands generated by two primers. Seven narrow-leafed lupin cultivars were distinguished using three other primers. Testing of RAPD marker polymorphism, supplemented in some cases with observations of seed coat color genes, allowed to identify all 12 yellow lupin cultivars. Thirteen field bean cultivars were tested by isozyme variability and RAPD polymorphism observations. Among 15 enzyme systems investigated, 10 showed a high level of inter- and intracultivar polymorphism but the range of allozyme variability discovered was useless for cultivar identification. All cultivars analysed could be distinguished by a combination of RAPD markers amplified using two primers.
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