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Secondary electron microscopy and transmission electron microscopy studies of carbon nanotubes in C-Ni films

100%

Kozłowski M., Dłużewski P., Kowalska E., Czerwosz E.

Open Physics

2011

vol. 9

issue 2

344-348

Carbon nanotubes films have been studied with SEM and TEM. The studied films were obtained using a two step method: PVD process and CVD process. Strongly defected and curled carbon nanotubes containing Ni nanoparticles formed the film with thickness of about 300–400 nm. Observed carbon nanotubes were of lengths from 100 nm to 300 nm and did not stick to each other.

Industrial diagnostics system using gamma radiation

100%

Jakowiuk A., Modzelewski Ł., Pieńkos J., Kowalska E.

issue 3

633-636

During the operation of large industrial installations, a very important task is to maintain the proper technical state. In the event of an emergency, it is vital to locate the place of occurrence as soon as possible. In solving this type of problem, it often helps to apply the methods of measurement associated with ionizing radiation. One of these methods is the gamma scanning. The purpose of this type of measurement is the detection and localization of disturbance of technological processes which may result in incorrect decomposition the fl owing medium and workpiece (sediments, congestion) as well as damage to the internal constructions. A particularly: (i) preventive diagnosis - early detection of installation failure; (ii) rationalization of repairs and renovations - to determine the need to take or not to take remedial action; (iii) quick and precise installation inspections - to gain knowledge of the technical condition and technological installations; (iv) indication of worn parts and posing a threat - diagnostics of the technical condition installation; (v) forecasting the useful lifetime of equipment.

Selection and analysis of a DNA aptamer binding α-amanitin from Amanita phalloides

64%

Muszyńska K., Ostrowska D., Bartnicki F., Kowalska E., Bodaszewska-Lubaś M., Hermanowicz P., Faulstich H., Strzałka W.

2017

vol. 64

issue 3

401-406

Mushroom foraging is very popular in some regions of the world. Sometimes toxic and edible mushrooms are mistaken by mushroom collectors, leading to serious human poisoning. The group of mushrooms highly dangerous for human health includes Amanita phalloides. This mushroom produces a toxic octapeptide called α-amanitin which is an inhibitor of nuclear RNA polymerase II. The inhibition of this polymerase results in the abortion of mRNA synthesis. The ingestion of A. phalloides causes liver failure due to the fact that most of the toxin is uptaken by hepatocytes. The hospitalization of poisoned patients involves the removal of the toxin from the digestive tract, its dilution in the circulatory system and the administration of therapeutic adjuvants. Since there is no effective antidote against amanitin poisoning, in this study we developed a DNA aptamer exhibiting specific binding to α-amanitin. This aptamer was selected using the SELEX (Systematic Evolution of Ligands by Exponential Enrichment) method. Next, its ability of toxin removal from aqueous solution was confirmed by pull-down assay. The aptamer region sufficient for α-amanitin binding was determined. Finally, the dissociation constant of the α-amanitin/DNA aptamer complex was calculated.

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1 Acta Biochimica Polonica

1 Nukleonika

1 Open Physics

1 2017

1 2015

1 2011

3 Kowalska E.

1 Bartnicki F.

1 Bodaszewska-Lubaś M.

1 Czerwosz E.

1 Dłużewski P.

1 Faulstich H.

1 Hermanowicz P.

1 Jakowiuk A.

1 Kozłowski M.

1 Modzelewski Ł.

1 Muszyńska K.

1 Ostrowska D.

1 Pieńkos J.

1 Strzałka W.

Search results

Secondary electron microscopy and transmission electron microscopy studies of carbon nanotubes in C-Ni films

Industrial diagnostics system using gamma radiation

Selection and analysis of a DNA aptamer binding α-amanitin from Amanita phalloides