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Developments of instruments and methods related with high-speed countercurrent chromatography and their applications in research of natural medicines

100%

Yao S., Cao Y., Jia C.-m., Wang Y., Song H.

Open Chemistry

2012

vol. 10

issue 3

417-432

High-speed countercurrent chromatography (HSCCC) is a liquid-liquid separation chromatographic technique which uses no solid supporting medium. During its rapid development in the last 30 years, great progress has been made in the instrumentation, the basic study and application of HSCCC. It has significant advantages over other instrumental separation techniques, in its high efficiency and continuous processing capability. In recent years, HSCCC has been widely used in research and development of natural medicines and functional foods, including preparative separation, fingerprint analysis and screening of bioactive constituents. A review of the technique is presented in this paper. [...]

Coffee consumption and risk of stroke: a meta-analysis of cohort studies

100%

Zhang R., Wang Y., Song B., Jørgensen H., Xu Y.

Open Medicine

2012

vol. 7

issue 3

310-316

Certain protein transducing agents convert translocated proteins into cell killers

73%

Tcherniuk S., Fiser A.-L., Derouazi M., Toussaint B., Wang Y., Wojtal I., Kondo E., Szolajska E., Chroboczek J.

2012

vol. 59

issue 3

433-439

The majority of proteins are unable to translocate into the cell interior. Hence for peptide- and protein-based therapeutics a direct intracytoplasmic delivery with the aid of transducing agents is an attractive approach. We wanted to deliver to the cell interior a putatively cytotoxic protein VPg. Protein transduction was achieved in vitro with three different commercial products. However, in our hands, delivery of various control proteins without known deleterious effects, as well as of protein VPg, always induced cell death. Finally, we used a novel transducing peptide Wr-T, which was not toxic to cultured cells, even in a quite large range of concentrations. Most importantly, control protein delivered to cells in culture did not display any toxicity while VPg protein exerted a strong cytotoxic effect. These data show that results obtained with cell-penetrating agents should be interpreted with caution.

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1 Acta Biochimica Polonica

1 Open Chemistry

1 Open Medicine

3 2012

3 Wang Y.

1 Cao Y.

1 Chroboczek J.

1 Derouazi M.

1 Fiser A.-L.

1 Jia C.-m.

1 Jørgensen H.

1 Kondo E.

1 Song B.

1 Song H.

1 Szolajska E.

1 Tcherniuk S.

1 Toussaint B.

1 Wojtal I.

1 Xu Y.

1 Yao S.

1 Zhang R.

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Developments of instruments and methods related with high-speed countercurrent chromatography and their applications in research of natural medicines

Coffee consumption and risk of stroke: a meta-analysis of cohort studies

Certain protein transducing agents convert translocated proteins into cell killers