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  1. 2 Biotechnologia

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  1. 2 2004

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  1. 2 Ilczuk A.

  2. 2 Witomska M.

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1

Effect of bulb chilling and growth regulators on the in vitro bulblet regeneration in Hippeastrum x chmielii Chm.

100%
Ilczuk A., Witomska M.
Biotechnologia
|
2004
|
issue 2
185-190
EN
Selected clones of a new intergeneric species Hippeastrum x chmielii Chm. grow vigorously, flower abundantly and produce flowers in clear, vivid colors. The hybrid is suitable both for cut flower production and as a pot plant. A great demand for planting the material is expected, therefore it is necessary to elaborate an efficient micropropagation method where due to application of growth regulators the bulblets can be differentiated from different tissues. To meet this goal, the trials were undertaken to propagate this hybrid from inflorescence shoots (scapes) obtained from bulbs (clone 2/7) chilled for 3, 5 or 7 months at 4oC. Both the length of chilling and the growth regulators in the MS medium affected bulblet regeneration. On the medium without growth regulators bulblets were scarce and appeared only on shoot explants from bulbs chilled for 5 months. The presence of 2 mg?dm-3 isopenthenyladenine (2iP) and 0,2 mg?dm-3 alpha-naphtylacetic acid (NAA), as well as combination of the same auxin with 2 mg?dm-3 benzyladenine (BA), stimulated bulblet formation on the explants. After 3 months of chilling, the regeneration was the poorest and the longer the chilling period was the more bulblets were produced on explants, but only in the presence of growth regulators.
2

Formation of adventitious bulblets in vitro on scale explants in Hippeastrum x chmielii Chm.

100%
Witomska M., Ilczuk A.
Biotechnologia
|
2004
|
issue 2
199-205
EN
Trials on micropropagation of a new interspecific hybrid Hippeastrum x chmielii are being carried out in the Department of Ornamental Plants at the Warsaw Agricultural University. In 2002-2003, an effect of BA on bulblet differentiation from explants of different size was tested. Initial material consisted of bulblets (5-6 mm of diameter, mean fresh weight 0,164 g) produced in vitro on MS medium without growth regulators. The bulblets were cut into halves or quarters along the main axis and placed polarly or apolarly on MS medium without growth regulators or with BA 1, 2, 4 or 8 mg?dm-3. Regeneration proceeded at 24oC under white, fluorescent light (24 mumol?m-2?s-1) for 16 hours and 8 hours of darkness. On the explants with basal part in the medium, the cytokinin limited a number of bulblets regenerated on both types of explants. The bulbs cut into quarters formed more bulblets than those cut in halves. When the explants were placed apicaly, the numbers of regenerated bulblets were similar in all the treatments. Fresh weight of bulblets regenerated on smaller explants (1/4 of a bulb) was higher when explants were placed polarly. Polarity did not affect the weight of bulblets produced on larger explants.
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