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Advances in regeneration and micropropagation

100%

Bach A.

Biotechnologia

1996

issue 1

83-93

The review discusses the recent advances in regeneration and micropropagation.The example include:1.new methods for enhancement of regeneration (etiolation of shoots, forced flushing, laser cutting of tissues, squashing and homogenization), 2.progress in preparing of media, 3.effect of electrostimulation or mycorrhizal fungi on plant micropropagation, 4.new system of mass propagation (advances in synthetic seed technology, micropropagation via SIT temporary immersion system), 5.novel application of plant tissue culture.

The effect of the exogenous phenolic compound, caffeic acid on organogenesis of Galanthus elwesii Hook. cultured in vitro

51%

Bach A., Pawlowska B., Hura K.

Biotechnologia

2010

issue 2

139-145

Caffeic acid is a simple phenolic compound, a phenylpropene derivative with two ?OH groups, which inhibits IAA oxidase and, therefore, is considered to be an auxin cofactor. The aim of the present experiments was to determine the effect of exogenous caffeic acid (100-500 mg dm-3 in medium) on the organogenesis in giant snowdrop (Galanthus elwesii) in in vitro cultures. Exogenous caffeic acid at the supra optimal concentration (500 mg dm-3) inhibited shoots, leaves and roots formation, as well as reduced bulbs fresh mass and caused the explants growing. The lower dose of caffeic acid inhibited the rhizogenesis process. The analysis of the content of phenolic compounds in tissues and organs of giant snowdrop showed that exogenous caffeic acid reduced total content of endogenic phenolic compounds in regenerants.

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2 Biotechnologia

1 2010

1 1996

2 Bach A.

1 Hura K.

1 Pawlowska B.

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Advances in regeneration and micropropagation

The effect of the exogenous phenolic compound, caffeic acid on organogenesis of Galanthus elwesii Hook. cultured in vitro