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1

Genetic diversity of inbred rye lines evaluated by RAPD analysis

100%
Myskow B., Masojc P., Banek-Tabor A., Szolkowski A.
Journal of Applied Genetics
|
2001
|
vol. 42
|
issue 1
1-14
EN
This study presents an attempt to supply breeders of hybrid rye with more genetic information on inbred lines, using molecular markers. Eighteen polymorphic loci detected by means of the RAPD (Random Amplified Polymorphic DNA) technique and mapped on 2R-7R rye chromosomes, were applied to study genetic similarities among forty inbred lines of rye. The lines were grouped in four main clusters revealed on dendrogram, which was generally consistent with the pedigree data. Mapped RAPD markers were shown to be a useful tool for phenetic studies in rye. Additionally, a system of 20 polymorphic fragments, detected by three primers, was developed for fingerprinting of rye lines. The system of RAPD markers, which was developed in this study, should be helpful in characterisation of rye genetic stocks used for breeding.
2

New genetic map of rye composed of PCR-based molecular markers and its alignment with the reference map of the DS2 ? RXL10 intercross

76%
Milczarski P., Banek-Tabor A., Lebiecka K., Stojalowski S., Myskow B., Masojc P.
Journal of Applied Genetics
|
2007
|
vol. 48
|
issue 1
11-24
EN
A new genetic map of rye, developed by using the 541 ? Ot1-3 F2 intercross, consists of 148 marker loci, including 99 RAPDs, 18 SSRs, 14 STSs, 9 SCARs and 7 ISSRs, and spans the distance of 1401.4 cM. To the 7 rye chromosomes, 8 linkage groups were assigned and compared with the reference map of the DS2 ? RXL10 F2 intercross by using 24 common markers. The 2 combined maps contain altogether 611 marker loci (70?109 per chromosome) and constitute a substantial source of information useful for further genomic studies in rye. From 21 to 37 RAPD marker loci are distributed randomly along each chromosome length and their total number for all 7 rye chromosomes is 177. This abundance of RAPD marker loci in the rye genetic map can be exploited for development of SCARs in regions containing important genes or QTL.
3

New genetic map of rye composed of PCR-based molecular markers and its alignment with the reference map of the DS2 ? RXL10 intercross

76%
Milczarski P., Banek-Tabor A., Lebiecka K., Stojalowski S., Myskow B., Masojc P.
Journal of Applied Genetics
|
2007
|
vol. 48
|
issue 3
11-24
EN
A new genetic map of rye, developed by using the 541 ? Ot1-3 F2 intercross, consists of 148 marker loci, including 99 RAPDs, 18 SSRs, 14 STSs, 9 SCARs and 7 ISSRs, and spans the distance of 1401.4 cM. To the 7 rye chromosomes, 8 linkage groups were assigned and compared with the reference map of the DS2 ? RXL10 F2 intercross by using 24 common markers. The 2 combined maps contain altogether 611 marker loci (70?109 per chromosome) and constitute a substantial source of information useful for further genomic studies in rye. From 21 to 37 RAPD marker loci are distributed randomly along each chromosome length and their total number for all 7 rye chromosomes is 177. This abundance of RAPD marker loci in the rye genetic map can be exploited for development of SCARs in regions containing important genes or QTL.
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