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1

The frequency of chromosomal XX/XY chimerism occurrence in Merino Booroola sheep

100%
Keszka J., Jaszczak K.
Journal of Applied Genetics
|
1996
|
vol. 37
|
issue 4
367-372
EN
Cytogenetic analysis was carried out in a total of 59 females and 45 males from 48 litters of Merino Booroola sheep. Karyotypes were investigated only in sheep coming from multiple heterosexual pregnancies. The study was performed over three consecutive years. Lymphocyte 54,XX/54,XY chimerism was identified in 16 ewes and 16 rams, i.e. 30.8% of the karyotyped group of animals and 10.32% of all animals born alive in the flock.
2

Chromosomal abnormalities in early embryos and commercial chicks and hatchability in reproduction flocks of layer and broiler hens

100%
Jaszczak K., Jaszczak J.
Genetica Polonica
|
1993
|
vol. 34
|
issue 3
287-293
EN
The frequency of chromosomal abnornalities in 16-18 hour embryos and one-day commercial chicks from parental flocks of layer (Astra S) and broiler (Astra B) hens as well as results of fertilization and hatchability in these flocks were compared. A karyotype analysis of Astra S showed that 6.5% of the embroys and 2.6% of the chicks were with chromosomal abnormalities. In the flock of Astra B hens only 3.0% of the embryos and 1.1% of the chicks had abnormal karyotype.Triploidy and chimerism were the most frequently identified abnormalities in both Astra A and Astra B flocks. No significant differences in the frequency of particular types of chromosomal aberrations were found between Astra S and Astra B flocks, except translocation and which were observed only among Astra S hens. The percentage of hatchability from set and fertile eggs in Astra S flock was 80.5 and 84.8 and that in Astra B was 84.3 and 88.3, respectively. The revealed differences in the frequency of chromosome aberrations and in the hatchability between the studied flocks of layer and broiler hens may result from genetic make-up of the strains and lines utilized for production of cross-bred chicken, such as the parental stocks of Astra S and Astra B.
3

Cytogenetic variation in farmed stock of Dybowski's sika deer

81%
Jaszczak K., Parada R., Krzywinski A.
Folia Biologica
|
2004
|
vol. 52
|
issue 1-2
53-56
EN
The chromosome constitution of Dybowski's sika deer was studied on the basis of 15 samples obtained from farmed stock maintained in an enclosure. The diploid chromosome number was 2n=68, 2n=67 and 2n=66. The constitutive heterochromatin (C-bands) was located in the centromeric regions of all acrocentric chromosomes. Metacentric chromosomes were C-negative. Chromosomes of three pairs proved to be NORs carriers. The size polymorphism of silver deposits was identified in two animals. A cytogenetic analysis indicated that the farmed stock of Dybowski's sika deer demonstrates considerable variation. The chromosome polymorphism observed may be a valuable marker for the management and preservation of this species.
4

Divergent selection for skeletal malformations in chickens alters polymorphism at microsatellite loci

71%
Wardecka B., Jaszczak K., Pierzchala M., Parada R., Korczak M.
Journal of Applied Genetics
|
2004
|
vol. 45
|
issue 1
61-71
EN
The objective of this study was to determine microsatellite polymorphism in Rhode Island Red (RIR) and Sussex (SX) chickens, divergently selected over six generations for high (H) or low (L) incidence of skeletal defects in embryos (30.7% for H lines, 3.7% for L lines). The polymorphism analysis covered 15 microsatellite markers within four lines (a total of 60 individuals). Eight alleles were identified as specific to H lines and six alleles as specific to L lines. The selection for skeletal malformation appears to have affected the frequency of microsatellite alleles. The experimental material examined constitutes a valuable source for identification of real genes causing skeletal defects.
5

Relationship between microsatellite marker alleles on chromosomes 1-5 originating from the Rhode Island Red and Green-legged Partrigenous breeds and egg production and quality traits in F2 mapping population

61%
Wardecka B., Olszewski R., Jaszczak K., Zieba G., Pierzchala M., Wicinska K.
Journal of Applied Genetics
|
2002
|
vol. 43
|
issue 3
319-329
EN
The objective of this study was to determine the relationship between the origin of marker alleles from the Rhode Island Red (RIR) and Green-legged Partrigenous (GlP) breeds and chosen egg production and quality traits in F2 generation consisting of 10 full-sib families. Polymorphism analysis of 23 microsatellite markers within the mapping population (519 F2) was made. In parental generation 17 alleles were identified as specific for the GlP and 23 for the RIR. The least squares method was used to evaluate the significance of effects of genotype (GlP/GlP, RIR/RIR, GlP/RIR) on the analysed quantitative traits. Thirty traits of egg production and quality were measured during the laying period. It was shown that the effects of the genotype (GlP/GlP, RIR/RIR, GlP/RIR) at the loci on analysed traits of F2 animals were diversified. Significant effects were found for 16 traits. These results confirm that the analysed microsatellite loci may be linked to the genes affecting egg production and quality traits. The loci examined and the experimental population constitutes a valuable material for QTL mapping (linkage analysis).
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