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  1. 1 2012

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  1. 1 Głód B.

  2. 1 Wantusiak P.

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Application of UV detection in HPLC in the total antioxidant potential assay

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Wantusiak P., Głód B.
Open Chemistry
|
2012
|
vol. 10
|
issue 6
1786-1790
EN
HPLC has been already used for the TAP estimation. Phenylalanine, salicylic, p-hydroxybenzoic (pHBA) or terephthalic (TPA) acids have been used as sensor compounds. Products of their reaction with the hydroxyl radicals, generated in the Fenton-like reaction, were analyzed using electrochemical or fluorescence detection. This paper describes the TAP assay based on the hydroxyl radicals reaction with pHBA, reversed-phase-HPLC separation and UV photometric detection. The elaborated assay has been used to evaluate TAP values of some apiculture products. [...]
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