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Development of antimicrobial packaging materials with immobilized glucose oxidase and lysozyme

100%
Hanušová K., Vápenka L., Dobiáš J., Mišková L.
Open Chemistry
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2013
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vol. 11
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issue 7
1066-1078
EN
Packaging based on immobilization of antimicrobial enzymes provides a promising form of active packaging systems applicable in food processing. Glucose oxidase and lysozyme were immobilized by the Ugi reaction with cyclohexyl isocyanide and glutaraldehyde on polyamide and ionomer films partially hydrolysed by hydrochloric acid. The immobilization of the enzymes on the surface of films was confirmed by FT-IR spectroscopy and the films were characterized by the specific activity of the immobilized enzymes. The enzyme migration into model solutions and the effect of pH, temperature and storage time on the activity of immobilized enzyme were also evaluated. Immobilization of lysozyme onto polyamide and ionomer films resulted in the loss of enzyme activity. The polyamide and ionomer films with immobilized glucose oxidase inhibited the growth of bacteria Escherichia coli CNCTC 6859, Pseudomonas fluorescens CNCTC 5793, Lactobacillus helveticus CH-1, Listeria ivanovii CCM 5884 and Listeria innocua CCM 4030 on agar media. [...]
2
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Determination of imazalil by on-line coupled capillary isotachophoresis with capillary zone electrophoresis

100%
Kvasnička F., Dobiáš J., Klaudisová-Chudáčková K.
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EN
A simple, rapid and reproducible capillary isotachophoretic on-line coupled with capillary zone electrophoresis (CITP-CZE) method for the determination of IMz in food packaging extracts and its residues in apples is described. A good separation of the IMZ from other sample constituents was achieved within 15 minutes without any sample clean up. Method characteristics (linearity, accuracy, intra-assay and detection limit) were determined. Less amount of time involved, sufficient sensitivity and low running cost are the important attributes of CITP-CZE method.
3
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Occurrence of 2-phenylphenol in food paper packages

88%
Votavová L., Hanušová K., Vápenka L., Dobiáš J., Kvasnička F.
Open Chemistry
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2014
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vol. 12
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issue 11
1162-1168
EN
Twenty three paper based packaging materials commercially used for food packaging in the Czech Republic were analysed with regard to 2-phenylphenol (OPP) content using HPLC technique with fluorimetric detection. The HPLC method was modified with the aim to increase its sensitivity. OPP was detected in quantities ranging from 10 mg kg−1 to 527 mg kg−1 in twenty tested samples. The level of OPP in packaging materials was in close correlation to the content of recycled pulp in used paper material. The migration of OPP from two packaging materials comprised of paperboard coated with low density polyethylene (LDPE) into food simulants was determined at 40°C for 10 days. The levels of migration into 3% acetic acid, 10% ethanol and olive oil were below the limit of detection of used analytical procedure. In the case of 95% ethanol, 13% and 21% of OPP present in tested samples were transferred into simulant. The found occurrence of OPP in paper packages does not pose any significant safety risk for food consumer, but OPP could be considered as wide spread contaminant in paper materials.
4
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Erratum to: “Occurrence of 2-phenylphenol in food paper packages”

88%
Votavová L., Hanušová K., Vápenka L., Dobiáš J., Kvasnička F.
Open Chemistry
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2014
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vol. 12
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issue 12
1304-1305
EN
The original version of the article was published in Cent. Eur. J. Chem. 12(11) (2014) pp. 1162–1168. Unfortunately, the original version of this article contains some mistakes in the Tables 2 and 3. Corrected versions of the tables are presented below.
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